Description

L-Lactic Dehydrogenase is a crucial oxidoreductase enzyme that catalyzes the interconversion of lactate and pyruvate with the concomitant reduction of NAD+ to NADH. This reaction is fundamental to cellular energy metabolism, making the enzyme an indispensable tool for biochemical research and diagnostic applications. It is essential for professionals in the pharmaceutical, clinical diagnostics, food & beverage, and biotechnology sectors who require reliable, high-activity enzymes for assay development, metabolic studies, and quality control testing.

Application

• Clinical Diagnostic Kits: A key component in enzymatic assays for measuring lactate, pyruvate, and alanine aminotransferase (ALT) levels in blood serum, vital for diagnosing conditions like myocardial infarction, liver disease, and hypoxia.
• Biochemical Research: Used extensively in vitro studies of glycolysis, gluconeogenesis, and cellular respiration to understand metabolic pathways and energy production.
• Pharmaceutical Quality Control: Employed in enzymatic methods for the purity testing and potency assessment of various pharmaceutical compounds.
• Food & Beverage Analysis: Utilized in enzymatic food testing kits to determine lactate concentration, which is an indicator of fermentation quality, spoilage, and acidity in products like dairy, meat, and wine.
• Biotechnology & Enzyme Engineering: Serves as a model enzyme for stability studies, immobilization techniques, and the development of biosensors.
• Academic & Teaching Laboratories: Provided as a standard reagent for educational experiments demonstrating enzyme kinetics and metabolic principles.

Basic Information

Product Name	L-Lactic Dehydrogenase
CAS No.	9001-60-9
Molecular Formula	N/A (Enzyme, Protein)
Molecular Weight	~140,000 Da (Tetramer)
Synonyms	LDH; L-Lactate Dehydrogenase; Lactate Dehydrogenase A; L-Lactate:NAD+ Oxidoreductase; EC 1.1.1.27; Heart LDH; Muscle LDH; Lactate NAD Oxidoreductase
EINECS	232-576-8

Quality Control

Our L-Lactic Dehydrogenase is produced and tested under a stringent quality management system. Each lot is characterized for specific activity, purity, and stability to ensure consistent performance in sensitive analytical applications. We provide comprehensive Certificates of Analysis (COA) with detailed specifications including activity units/mg protein, pH optimum, temperature stability, and absence of interfering enzymes. Our quality commitment aligns with the standards expected for in-vitro diagnostic (IVD) and research-grade reagents.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent activity loss. The product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	> 500 units/mg protein (at 37°C, pH 7.5)
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	> 95%
pH Optimum	7.0 - 7.5
Temperature Optimum	37°C
Stabilizers	May contain BSA or sucrose
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.