Description

Hydroxysteroid Dehydrogenase is a critical enzyme belonging to the oxidoreductase class, specifically catalyzing the reversible oxidation/reduction of hydroxyl groups on steroid molecules. This biochemical activity is fundamental for research and industrial processes involving steroid hormone metabolism and synthesis. It is an essential reagent for pharmaceutical development, clinical diagnostics, and biochemical research, particularly in endocrinology and metabolic studies.

Application

• Pharmaceutical Intermediates Synthesis: Key biocatalyst in the production of steroid-based active pharmaceutical ingredients (APIs) and intermediates.
• Clinical Diagnostic Kits: Used as a core enzyme component in enzymatic assays for quantifying steroid hormones (e.g., cortisol, testosterone, estradiol) in serum and urine.
• Biochemical Research: Essential tool for studying steroid metabolism pathways, enzyme kinetics, and hormone regulation in academic and industrial R&D laboratories.
• Biotransformation Processes: Employed in enzymatic conversion steps for the selective modification of steroid substrates in green chemistry applications.
• Quality Control Testing: Used in the pharmaceutical industry for the analysis and purity assessment of steroid compounds.

Basic Information

Product Name	Hydroxysteroid Dehydrogenase
CAS No.	9001-56-3
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by source and subunit composition
Synonyms	3α-Hydroxysteroid Dehydrogenase; 3β-Hydroxysteroid Dehydrogenase; 17β-Hydroxysteroid Dehydrogenase; HSD; Steroid Dehydrogenase; 3α-HSD; 3β-HSD; 17β-HSD; EC 1.1.1.50/51/52 etc.
EINECS	Contact for details

Quality Control

Our Hydroxysteroid Dehydrogenase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and stability. Certificates of Analysis (COA) detailing activity units/mg protein, purity by SDS-PAGE, and functional performance are provided. We adhere to relevant guidelines for research and diagnostic enzyme production.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Aliquot upon receipt for optimal preservation of enzymatic activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms (Specific activity provided on COA)
Specific Activity	> X U/mg protein (Grade dependent)
Purity (SDS-PAGE)	> 90% (Single major band)
Protein Content	Determined by Bradford/Lowry method
Stabilizers/Additives	May contain buffer salts (e.g., phosphate, Tris) or glycerol
Storage Buffer	Specified on label and COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.