Description

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a crucial enzyme in the glycolytic pathway, catalyzing the oxidative phosphorylation of glyceraldehyde 3-phosphate. Its reliable activity is essential for fundamental research in cellular metabolism, energy production, and associated disease mechanisms. This high-purity enzyme is indispensable for biopharmaceutical R&D, diagnostic kit manufacturing, and academic research in biochemistry and molecular biology.

Application

• Biochemical Research & Assay Development: A key reagent for studying glycolysis, gluconeogenesis, and cellular energetics in vitro.
• Diagnostic Manufacturing: Used as a core component in enzymatic assay kits for clinical diagnostics and research use.
• Pharmaceutical R&D: Employed in target validation and screening assays for metabolic disorders, cancer, and neurodegenerative diseases.
• Enzyme Activity Standard: Serves as a reference standard for calibrating equipment and validating other enzymatic assays.
• Educational & Teaching Kits: A fundamental enzyme used in university-level laboratory courses to demonstrate metabolic pathways.

Basic Information

Product Name	Glyceraldehyde-3-Phosphate Dehydrogenase
CAS No.	9001-50-7
Molecular Formula	N/A (Protein)
Molecular Weight	~145 kDa (Tetramer)
Synonyms	GAPDH; Glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating); D-Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde phosphate dehydrogenase; Triosephosphate dehydrogenase; NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase; EC 1.2.1.12
EINECS	232-628-4

Quality Control

Our Glyceraldehyde-3-Phosphate Dehydrogenase is manufactured under strict quality systems. Each lot undergoes comprehensive testing to ensure high specific activity, purity, and stability. We provide detailed Certificates of Analysis (COA) with key parameters including activity (U/mg protein), purity level (by SDS-PAGE), and absence of contaminating enzymatic activities. Production can adhere to relevant guidelines for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, store at 2-8°C. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ 100 U/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	10 - 20 mg/mL (as supplied)
Stabilizers	Potassium phosphate, EDTA, DTT (or similar)
Activity Definition	One unit reduces 1.0 μmol of NAD+ per minute at pH 8.9 and 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.