Description

Glutamate Dehydrogenase (CAS 9001-46-1) is a critical enzyme that catalyzes the reversible oxidative deamination of L-glutamate to α-ketoglutarate and ammonia. This reaction is fundamental to nitrogen metabolism, making the enzyme an indispensable tool for biochemical research and diagnostic applications. It is essential for professionals in the pharmaceutical, biotechnology, and clinical diagnostics industries who require high-purity, reliable enzymes for assay development, metabolic studies, and quality control testing.

Application

• Clinical Diagnostic Kits: A key component in enzymatic assays for the quantitative determination of ammonia, urea, and L-glutamate in blood serum and other biological fluids.
• Biochemical Research: Used as a reagent in metabolic pathway studies, enzyme kinetics investigations, and research into nitrogen assimilation and amino acid metabolism.
• Pharmaceutical Quality Control: Employed in enzymatic methods for the assay of related compounds and the monitoring of specific biochemical reactions during drug development and manufacturing.
• Food & Beverage Analysis: Utilized in enzymatic test kits for the determination of glutamate content, an important flavor enhancer and quality indicator.
• Biocatalyst Development: Investigated for potential use in biocatalytic processes for the synthesis of fine chemicals and chiral intermediates.
• Educational & Teaching Laboratories: Serves as a standard enzyme for demonstrating enzymatic principles and assay techniques in academic settings.

Basic Information

Product Name	Glutamate Dehydrogenase
CAS No.	9001-46-1
Molecular Formula	Protein (Enzyme)
Molecular Weight	~330,000 Da (Hexamer)
Synonyms	GDH; L-Glutamate Dehydrogenase; Glutamic Dehydrogenase; NAD(P)+-Glutamate Dehydrogenase; EC 1.4.1.3; Dehydrogenase, Glutamate; L-Glutamate:NAD(P)+ Oxidoreductase (Deaminating)
EINECS	232-579-0

Quality Control

Our Glutamate Dehydrogenase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and stability. We provide detailed Certificates of Analysis (COA) that include data on activity, protein content, and impurity profiles. While not of pharmacopoeial grade unless specified, our standard material is suitable for research and industrial applications, with custom purification levels available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect activity. Avoid repeated freeze-thaw cycles of prepared solutions.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Identification (Activity)	Conforms
Specific Activity	≥ 40 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers	May contain buffer salts (e.g., phosphate) and glycerol
pH (in solution)	7.0 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.