Description

β-Glucuronidase is a hydrolytic enzyme that specifically cleaves the glycosidic bond of glucuronides, releasing free aglycones and glucuronic acid. This catalytic activity is critical for analytical sample preparation, diagnostic assays, and biochemical research where the quantification of specific compounds is required. It is an essential reagent for laboratories and manufacturers in the pharmaceutical, clinical diagnostics, and food safety testing industries.

Application

• Clinical Diagnostics & Toxicology: Hydrolysis of drug-glucuronide conjugates (e.g., opiates, benzodiazepines) in urine and serum samples prior to analysis by GC-MS or HPLC.
• Pharmaceutical Research: Enzymatic hydrolysis in drug metabolism and pharmacokinetic (DMPK) studies to investigate phase II conjugation pathways.
• Food & Environmental Analysis: Deconjugation of mycotoxin-glucuronides (e.g., deoxynivalenol, zearalenone) and estrogenic compounds in complex matrices for accurate quantification.
• Biochemical Assay Kits: A key component in enzymatic assay kits for the detection of specific hormones, neurotransmitters, or metabolites.
• Forensic Science: Sample pretreatment for the confirmatory analysis of drugs of abuse in forensic toxicology.
• Biotechnology: Used in the synthesis and modification of complex carbohydrates and glycoconjugates.

Basic Information

Item	Detail
Product Name	β-Glucuronidase
CAS No.	9001-45-0
Molecular Formula	N/A (Enzyme)
Molecular Weight	~280-300 kDa (Varies by source)
Synonyms	β-Glucuronidase; β-Glucuronidase; Glucuronidase; β-D-Glucuronoside glucuronosohydrolase; EC 3.2.1.31; Ketodase; Glucurase; Bacterial β-Glucuronidase (from E. coli); Helix Pomatia β-Glucuronidase
EINECS	232-620-3

Quality Control

Our β-Glucuronidase is manufactured and tested under a rigorous quality management system. Each batch is characterized for key parameters including specific activity, purity, and absence of interfering enzymatic activities. Certificates of Analysis (COA) detailing assay results, storage conditions, and stability data are provided to ensure traceability and compliance with your application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, avoid repeated freeze-thaw cycles of liquid preparations and store lyophilized powder under desiccant.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (Activity)	Conforms
Specific Activity	>100,000 U/mg protein (Type dependent)
Purity (SDS-PAGE)	>90%
pH (in solution)	6.8 - 7.2
Protein Content (Biuret)	Reported on COA
Bacterial Endotoxins (LAL)	<1.0 EU/mg (For diagnostic grades)
Microbial Limit	Absence of specified pathogens

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.