Description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a critical oxidoreductase enzyme that catalyzes the first and rate-limiting step of the pentose phosphate pathway. This reaction is fundamental for maintaining cellular redox balance and generating NADPH, a key reducing agent for biosynthetic processes and antioxidant defense. It is an essential reagent for diagnostic, research, and industrial applications. Key industries that rely on this high-purity enzyme include in-vitro diagnostics (IVD), pharmaceutical research, and biochemical manufacturing.

Application

• Clinical Diagnostics: Core component in enzymatic assay kits for the quantitative determination of glucose, ATP, and hexokinase activity in blood and serum samples.
• Biochemical Research: Essential tool for studying carbohydrate metabolism, oxidative stress, NADPH-dependent pathways, and erythrocyte biology.
• Pharmaceutical Development: Used in enzyme-coupled assays for high-throughput screening (HTS) of drug candidates targeting metabolic disorders.
• Food & Beverage Analysis: Employed in enzymatic methods for the accurate measurement of glucose and related sugars in food products.
• Quality Control (QC) Reagents: Integral to reagent formulations used in clinical chemistry analyzers and laboratory instrumentation.
• Enzyme Manufacturing: Serves as a starting material or reference standard for the production of diagnostic and research-grade enzyme blends.

Basic Information

Product Name	Glucose-6-Phosphate Dehydrogenase
CAS No.	9001-40-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	~50-60 kDa (Subunit); Variable multimer (Native)
Synonyms	G6PD; D-Glucose-6-phosphate:NADP+ 1-oxidoreductase; NADP+-dependent Glucose-6-phosphate Dehydrogenase; Zwischenferment; G-6-PD; Glucose 6 Phosphate Dehydrogenase; EC 1.1.1.49
EINECS	232-602-5

Quality Control

Our Glucose-6-Phosphate Dehydrogenase is manufactured under strict quality systems. Each lot is tested to ensure high specific activity, purity, and stability, meeting the rigorous standards required for diagnostic and research applications. Certificates of Analysis (COA) are provided with each shipment, detailing key parameters such as activity, protein content, and purity. We support compliance with relevant regulatory frameworks, including ISO 13485 for IVD manufacturers.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or suspension
Specific Activity	≥ 200 U/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers	Ammonium Sulfate, EDTA, DTT (as applicable)
pH (in solution)	7.0 - 8.0
Solubility	Soluble in water or buffer
Biological Source	Leuconostoc mesenteroides (common)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.