Description

Collagenase is a proteolytic enzyme complex specifically designed to hydrolyze native collagen and gelatin. This enzymatic activity is critical for breaking down the structural proteins in connective tissue, making it an indispensable tool in various biotechnological and medical processes. It is essential for researchers and manufacturers in the pharmaceutical, biomedical research, and tissue engineering industries who require precise and efficient tissue dissociation.

Application

• Tissue Dissociation and Cell Isolation: Primary application for isolating viable cells from various tissues (e.g., liver, heart, adipose) for research and cell culture.
• Pharmaceutical Manufacturing: Used in the production of certain injectable drugs and in wound debridement products.
• Biomedical Research: Essential for creating organoid models, studying extracellular matrix (ECM) biology, and investigating cell-matrix interactions.
• Tissue Engineering: Employed to decellularize tissues and organs, providing scaffolds for regenerative medicine applications.
• Diagnostics: Used in sample preparation for histological analysis and certain diagnostic assays.
• Industrial Processing: Applied in the leather industry for bating and in the food industry for tenderization processes (specific food-grade variants).

Basic Information

Product Name	Collagenase
CAS No.	9001-12-1
Molecular Formula	Protein (Enzyme Complex)
Molecular Weight	Varies (Approx. 68-130 kDa for different isoforms)
Synonyms	Clostridiopeptidase A; Clostridium histolyticum collagenase; Achromobacter iophagus collagenase; Collagenase Type I, II, IV, etc.; Microbial collagenase; Matrix metalloproteinase-1 (MMP-1) analog; Tissue dissociation enzyme; CAS 9001-12-1
EINECS	232-582-9

Quality Control

Our Collagenase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and absence of contaminating non-collagenolytic proteases. We provide comprehensive Certificates of Analysis (COA) detailing potency (CDU/mg or AU/mg), sterility (where applicable), and endotoxin levels. Production can adhere to relevant guidelines for research-use or current Good Manufacturing Practice (cGMP) standards for clinical-grade material.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (cold chain). This product is hygroscopic (moisture-sensitive). For long-term stability, desiccate at -20°C. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Hydrolyzes collagen substrate
Assay (Specific Activity)	≥ 0.5 - 5.0 CDU/mg (or as per grade)
Purity (HPLC)	≥ 90% (or as per grade)
pH (in solution)	6.0 - 7.5
Endotoxin	< 10 EU/mg (for in-vivo grades)
Microbial Limits	Meets specified bioburden requirements

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.