Description

Aspartate Aminotransferase (AST) is a critical pyridoxal phosphate-dependent enzyme that catalyzes the reversible transfer of an amino group between aspartate and α-ketoglutarate to form oxaloacetate and glutamate. This reaction is fundamental to cellular amino acid metabolism and the malate-aspartate shuttle. As a key diagnostic biomarker, it is essential for clinical laboratories, pharmaceutical manufacturers, and research institutions focused on liver and cardiac function, metabolic studies, and in vitro diagnostics (IVD) reagent production.

Application

• Clinical Diagnostics: Primary component in reagent kits for the quantitative determination of serum AST (SGOT) levels, a standard test for liver and heart health assessment.
• Biochemical Research: Used as a tool enzyme in studies of amino acid metabolism, transamination pathways, and mitochondrial function.
• Pharmaceutical Development: Employed in enzymatic assays for drug metabolism and toxicity screening studies.
• In Vitro Diagnostic (IVD) Manufacturing: A raw material for the production of calibrators, controls, and liquid-stable or dry-powder clinical chemistry reagents.
• Food & Beverage Analysis: Potential use in enzymatic methods for food quality control and fermentation process monitoring.
• Educational Kits: Supplied for teaching principles of enzymology and clinical biochemistry in academic settings.

Basic Information

Product Name	Aspartate Aminotransferase
CAS No.	9000-97-9
Molecular Formula	N/A (Enzyme)
Molecular Weight	~90-100 kDa (Dimer, varies by source)
Synonyms	AST; Glutamic-Oxaloacetic Transaminase (GOT); Serum Glutamic-Oxaloacetic Transaminase (SGOT); Aspartate Transaminase; L-Aspartate:2-Oxoglutarate Aminotransferase; EC 2.6.1.1; Transaminase A
EINECS	Contact for details

Quality Control

Our Aspartate Aminotransferase is manufactured under strict quality systems. Each lot is tested to ensure high specific activity, purity, and consistency suitable for diagnostic and research applications. We provide comprehensive Certificates of Analysis (COA) detailing parameters such as activity, protein content, and impurity profiles. Production can adhere to relevant ISO 13485 standards for IVD use upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, keep desiccated. Avoid repeated freeze-thaw cycles of reconstituted solutions.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	> 100 U/mg protein
Protein Content (Biuret)	Contact for details
Activity Ratio (AST/ALT)	< 0.1%
pH (in solution)	6.0 - 7.5
Solubility	Clear solution in buffer
Loss on Drying	< 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.