Description

Bicinchoninic Acid Disodium Salt is a high-purity, water-soluble reagent essential for the quantitative colorimetric determination of proteins. Its core value lies in forming a stable, purple-colored complex with cuprous ions (Cu¹⁺) in the presence of peptides, which is the fundamental principle of the widely adopted BCA (bicinchoninic acid) protein assay. This compound is critical for researchers and quality control laboratories requiring accurate, sensitive, and reproducible protein quantification. Key industries that rely on this reagent include biotechnology, pharmaceuticals, diagnostic manufacturing, and academic research institutions.

Application

• BCA Protein Assay Reagent: Primary component in solution A for the colorimetric quantification of total protein concentration.
• Biopharmaceutical Development: Used in process development and quality control for measuring protein content in drug substances and products.
• Diagnostic Kit Manufacturing: Integral to the formulation of commercial diagnostic kits and reagents for clinical chemistry analyzers.
• Cell Biology & Biochemistry Research: Standard method for protein analysis in cell lysates, tissue homogenates, and purified protein samples.
• Food & Beverage Analysis: Applied in quality control labs to determine protein content in nutritional products and raw materials.
• Antibody & Enzyme Production: Used to monitor protein yield and concentration during purification and formulation processes.

Basic Information

Product Name	Bicinchoninic Acid Disodium Salt
CAS No.	979-88-4
Molecular Formula	C₂₂H₁₀N₂Na₂O₄
Molecular Weight	388.30 g/mol
Synonyms	BCA Disodium Salt; 2,2'-Biquinoline-4,4'-dicarboxylic Acid Disodium Salt; Disodium Bicinchoninate; Bicinchoninic Acid Sodium Salt; BCA Na₂; 4,4'-Dicarboxy-2,2'-biquinoline Disodium Salt; Protein Assay Reagent BCA
EINECS	213-562-1

Quality Control

Our Bicinchoninic Acid Disodium Salt is manufactured under a strict quality management system to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and UV-Vis spectroscopy, and stringent limits for moisture and heavy metals. A detailed Certificate of Analysis (COA) providing lot-specific data is available for every shipment to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed after each use to prevent absorption of atmospheric moisture, which can affect performance.

Specification

Item	Specification
Appearance	Light yellow to beige powder
Identification (IR)	Conforms to reference spectrum
Identification (UV-Vis)	Conforms
Purity (HPLC)	≥ 98.0%
Water (Karl Fischer)	≤ 5.0%
Heavy Metals (as Pb)	≤ 20 ppm
Assay (on dried basis)	97.0 - 103.0%
Performance (BCA Assay)	Meets functional test criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.