Description

4-[2,2-Bis[(p-Tolylsulfonyl)-Methyl]Acetyl]Benzoic Acid-n-Hydroxy Succinimidyl Ester is a high-purity, activated ester derivative designed for efficient bioconjugation and crosslinking applications. This compound serves as a critical reagent for the covalent attachment of molecules containing carboxylic acid groups to primary amines, enabling the synthesis of stable amide bonds under mild conditions. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and advanced materials science who require reliable and specific coupling agents for creating novel biomolecular constructs and diagnostic conjugates.

Application

• Bioconjugation Reagent: For coupling carboxylic acid-containing ligands, peptides, or haptens to carrier proteins (e.g., BSA, KLH) for antibody production and immunoassay development.
• Crosslinker for Biomaterials: Used in the functionalization of surfaces, hydrogels, and polymeric scaffolds to immobilize bioactive molecules for tissue engineering and biosensor applications.
• Pharmaceutical Intermediate: Serves as a key building block in the synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutic agents.
• Proteomics Research: Facilitates the labeling and modification of proteins and peptides for mass spectrometry analysis, protein interaction studies, and activity-based profiling.
• Diagnostic Kit Manufacturing: Employed in the production of enzyme conjugates and labeled antibodies for ELISA, lateral flow assays, and other clinical diagnostic platforms.
• Polymer Modification: Enables the introduction of functional amine-reactive groups into polymer chains for creating advanced functional materials.

Basic Information

Product Name	4-[2,2-Bis[(p-Tolylsulfonyl)-Methyl]Acetyl]Benzoic Acid-n-Hydroxy Succinimidyl Ester
CAS No.	899452-51-8
Molecular Formula	C32H29NO9S2
Molecular Weight	635.70 g/mol
Synonyms	4-[2,2-Bis[(4-Methylphenyl)sulfonyl)methyl]acetyl]benzoic acid N-hydroxysuccinimide ester; 4-[2,2-Bis(p-tolylsulfonylmethyl)acetyl]benzoic acid NHS ester; N-Hydroxysuccinimide ester of 4-[2,2-bis[(p-tolylsulfonyl)methyl]acetyl]benzoic acid; 4-(2,2-Bis((4-methylphenyl)sulfonyl)methyl)acetyl)benzoic acid 2,5-dioxopyrrolidin-1-yl ester; NHS-activated bis-sulfone ester; Crosslinking agent 899452-51-8; Activated ester crosslinker
EINECS	Contact for details

Quality Control

Our 4-[2,2-Bis[(p-Tolylsulfonyl)-Methyl]Acetyl]Benzoic Acid-n-Hydroxy Succinimidyl Ester is manufactured under strict quality management systems to ensure batch-to-batch consistency and high reactivity. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment, NMR and MS for structural confirmation, and functional testing to verify conjugation efficiency. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research and cGMP-compliant manufacturing needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, freezer environment. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.