Description

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-Nh2 is a synthetic, nonapeptide derivative containing a 2,4-dinitrophenyl (Dnp) quencher group, making it a valuable tool for fluorescence resonance energy transfer (FRET) studies and protease activity assays. This compound matters for researchers and developers requiring a specific, high-purity substrate to study enzyme kinetics, particularly for matrix metalloproteinases (MMPs) and other proteolytic enzymes. It is primarily needed by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in drug discovery and biochemical research. The product is supplied with a Certificate of Analysis to ensure batch-to-batch consistency for critical experimental work.

Application

• Protease Substrate: Used as a selective FRET-based substrate for monitoring the activity of various matrix metalloproteinases (MMPs) and other proteases in vitro.
• Enzyme Kinetic Studies: Essential for determining kinetic parameters (Km, Vmax, kcat) of proteolytic enzymes in high-throughput screening (HTS) and mechanistic studies.
• Drug Discovery & Screening: Employed in pharmaceutical R&D to identify and characterize novel protease inhibitors for therapeutic applications.
• Biochemical Assay Development: Serves as a key component in developing sensitive and specific fluorescence-quenching assays for diagnostic or research purposes.
• Cell Biology Research: Utilized to study protease function and localization in cellular pathways and disease models, such as cancer metastasis and inflammation.
• Quality Control in Biologics: Applied in the quality assessment of enzyme-based biopharmaceuticals to ensure consistent potency and activity.

Basic Information

Product Name	Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-Nh2
CAS No.	891198-38-2
Molecular Formula	C55H73N13O21
Molecular Weight	1244.24 g/mol
Synonyms	Mca-PLGLGEA-Dap(Dnp)-NH2; (7-Methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Lys(Dnp)-NH2; FRET Substrate for MMPs; Mca-PLGLGEAK(Dnp)-NH2; MMP Substrate III; Fluorogenic Peptide Substrate Mca-PLGL-Dpa-AR-NH2 (related); Dnp-Quenched FRET Peptide; Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Lys(Dnp)-NH2; Protease Fluorescence Substrate 891198-38-2
EINECS	Contact for details

Quality Control

Every batch of Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-Nh2 is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance. Our standard quality commitment includes comprehensive analysis via HPLC, MS, and NMR to confirm the structure and achieve high purity levels suitable for sensitive research applications. Certificates of Analysis (COA) detailing batch-specific results are provided and available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 80%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.