Description

Ac-Gly-Pro-Afc CAS NO 886993-02-8 is a specialized fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of specific protease activity. It is primarily utilized in the pharmaceutical and life sciences industries for high-throughput screening, biochemical research, and diagnostic development.

Application

• Protease Activity Assays: Used as a selective substrate for measuring the activity of dipeptidyl peptidases (DPP) and related proteases in research settings.
• High-Throughput Screening (HTS): Enables rapid, fluorometric screening of compound libraries for protease inhibitors in drug discovery programs.
• Biochemical Research: Serves as a key tool for studying enzyme kinetics, mechanism of action, and substrate specificity of proteolytic enzymes.
• Diagnostic Development: Employed in the development of clinical diagnostic kits for conditions linked to abnormal protease activity.
• Cell-Based Assays: Facilitates the monitoring of protease activity within live cells or cell lysates for functional studies.
• Quality Control in Biologics: Applied in the purity and potency testing of therapeutic enzymes and biopharmaceutical products.

Basic Information

Product Name	Ac-Gly-Pro-Afc
CAS No.	886993-02-8
Molecular Formula	C₂₀H₂₄FN₅O₆
Molecular Weight	449.43 g/mol
Synonyms	Ac-Gly-Pro-AMC (common alternate); Acetyl-Glycyl-Prolyl-7-Amino-4-Trifluoromethylcoumarin; N-Acetyl-Gly-Pro-AFC; DPP IV Substrate Ac-Gly-Pro-AFC; Fluorogenic DPP-IV Substrate; Ac-GP-AFC; Ac-Gly-Pro-AFC trifluoroacetate salt (if applicable)
EINECS	Contact for details

Quality Control

Our Ac-Gly-Pro-Afc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.