Description

4-Methylumbelliferyl-β-D-Glucuronid Hydrat 4-Methylumbelliferyl-β-D-Glucuronide Hydrate is a high-purity fluorogenic substrate used for the detection and quantification of β-glucuronidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by biotechnology, pharmaceutical, and clinical diagnostic laboratories for enzyme-linked assays, microbial detection, and reporter gene studies.

Application

• Clinical Diagnostics: As a substrate for detecting β-glucuronidase activity in assays for pathogens like E. coli in water and food safety testing.
• Pharmaceutical Research: For high-throughput screening (HTS) of compounds that modulate β-glucuronidase activity in drug discovery.
• Molecular Biology: In reporter gene assays (e.g., GUS assays) to study gene expression and promoter activity in plant and microbial systems.
• Microbiology: For the identification and enumeration of β-glucuronidase-positive bacteria in industrial and environmental samples.
• Biochemical Research: As a standard in enzyme kinetics studies to determine kinetic parameters (Km, Vmax) of β-glucuronidase.
• Quality Control: Used in the manufacturing and QC of diagnostic kits and reagents that rely on fluorogenic enzyme substrates.

Basic Information

Product Name	4-Methylumbelliferyl-β-D-Glucuronid Hydrat 4-Methylumbelliferyl-β-D-Glucuronide Hydrate
CAS No.	881005-91-0
Molecular Formula	C16H16O9 • xH2O
Molecular Weight	352.29 g/mol (anhydrous basis)
Synonyms	4-Methylumbelliferyl β-D-Glucuronide Hydrate; 4-MUG; 4-Methylumbelliferyl-β-D-glucopyranosiduronic Acid Hydrate; MUG Hydrate; 4-MU-β-D-Glucuronide; β-Glucuronidase Substrate; 7-Hydroxy-4-methylcoumarin β-D-glucuronide; GUS Substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-β-D-Glucuronide Hydrate is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. Due to its hygroscopic nature, the container should be allowed to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Conforms (Functional Test)
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.