Description

Biotin-Peg4-N3 CAS NO 875770-34-6 is a high-purity, heterobifunctional crosslinking reagent featuring a biotin group for affinity capture, a tetraethylene glycol (PEG4) spacer for enhanced solubility, and an azide (N3) moiety for copper-free click chemistry. This compound is critical for researchers and developers requiring precise, bioorthogonal conjugation strategies to label, capture, or modify biomolecules and surfaces. It is primarily utilized in the fields of bioconjugation, proteomics, drug discovery, and diagnostic assay development, where reliable and efficient linker chemistry is paramount.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, enzymes, and other proteins via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Proteomics and Pull-Down Assays: Immobilization of bait proteins or small molecules onto streptavidin/avidin resins for affinity purification and interaction studies.
• Diagnostic Kit Development: Conjugation of detection probes (e.g., fluorescent dyes, enzymes) to targeting molecules for ELISA, lateral flow, and other immunoassays.
• Drug Delivery System Research: Functionalization of nanoparticles, liposomes, or polymer carriers for targeted delivery and subsequent analysis.
• Cell Surface Engineering: Labeling and tracking of cell surface receptors or glycans through metabolic incorporation of azide-modified sugars followed by biotinylation.
• Materials Science: Creating biofunctionalized surfaces and hydrogels for biosensor applications and tissue engineering scaffolds.

Basic Information

Item	Details
Product Name	Biotin-Peg4-N3
CAS No.	875770-34-6
Molecular Formula	C27H48N8O8S
Molecular Weight	644.79 g/mol
Synonyms	Biotin-PEG4-azide; Biotin-PEG4-N3; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl)pentanamide; Azido-PEG4-biotin; N-(Azido-PEG4)-biotin; Biotinylated PEG azide; Biotin-PEG4-azide crosslinker
EINECS	Contact for details

Quality Control

Every batch of Biotin-Peg4-N3 is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We provide a Certificate of Analysis (COA) with each shipment, detailing purity, identity, and impurity profiles. Our quality commitment ensures the product meets the stringent requirements for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.