Description

n-Succinimidyl-S-Acetylthiohexanoate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a protected thiol group. This compound is critical for creating stable, site-specific conjugates between proteins, peptides, or other biomolecules containing primary amines and thiol-reactive surfaces or molecules. It is primarily utilized by researchers and manufacturers in the bioconjugation, diagnostics, and pharmaceutical development sectors who require controlled and efficient linker chemistry.

Application

• Bioconjugation and Protein Labeling: For covalently attaching fluorescent dyes, enzymes, or other probes to antibodies, proteins, or peptides via amine groups, with subsequent deprotection for thiol-based coupling.
• Surface Functionalization: Immobilization of biomolecules onto gold surfaces, maleimide-activated particles, or other thiol-reactive substrates for biosensor and microarray development.
• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a component in the synthesis of cleavable or non-cleavable linkers for connecting cytotoxic payloads to monoclonal antibodies.
• Polymer Modification: Introduction of protected thiol handles into amine-containing polymers for subsequent click chemistry or further functionalization.
• Diagnostic Reagent Manufacturing: Production of enzyme-linked or fluorescently-labeled detection reagents for immunoassays (ELISA, lateral flow tests).
• Peptide Synthesis and Modification: Used in SPPS or solution-phase synthesis to introduce a protected thiol functionality onto peptide sequences.

Basic Information

Product Name	n-Succinimidyl-S-Acetylthiohexanoate
CAS No.	874743-76-7
Molecular Formula	C12H17NO5S
Molecular Weight	287.33 g/mol
Synonyms	S-Acetyl-6-mercaptohexanoic acid N-succinimidyl ester; 6-[(Acetylthio)hexanoic acid] 2,5-dioxopyrrolidin-1-yl ester; SATP; NHS-SATP; N-Succinimidyl S-acetyl-6-thiohexanoate; Acetylthio-PEG5-NHS ester; Acetylthio-PEG5-OSu; SATA (6-carbon chain analog)
EINECS	Contact for details

Quality Control

Our n-Succinimidyl-S-Acetylthiohexanoate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your research and cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.