Description

5-Bromo-6-Chloro-3-Indoxyl Butyrate is a specialized chromogenic substrate used in advanced biochemical and diagnostic applications. This compound is critical for the detection and quantification of specific enzymatic activities, offering high sensitivity and selectivity. It is primarily utilized by research institutions and manufacturers in the life sciences, pharmaceutical development, and diagnostic kit production sectors.

Application

• Enzyme Substrate for Diagnostic Assays: Key component in chromogenic tests for detecting β-galactosidase and related hydrolase enzymes.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ) to visualize and quantify gene expression in cell cultures and tissues.
• Histochemistry & Pathology: Employed in tissue staining protocols to localize specific enzymatic activity at the cellular level.
• Microbiology: Applied in microbial identification systems to differentiate bacterial species based on enzymatic profiles.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify enzyme inhibitors or activators for drug discovery.
• Biochemical Reagent Manufacturing: Serves as a raw material for producing ready-to-use diagnostic reagents and kits.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indoxyl Butyrate
CAS No.	873295-29-5
Molecular Formula	C12H11BrClNO3
Molecular Weight	332.57 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl butyrate; BCIP Butyrate; 3-Indoxyl butyrate, 5-bromo-6-chloro-; 5-Bromo-6-chloro-1H-indol-3-yl butanoate; X-BCIP; Chromogenic BCIP substrate; 5-Br-6-Cl-3-indoxyl butyrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indoxyl Butyrate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive diagnostic and research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our customers' compliance needs in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.