Description

N4-Diisobutylaminomethylidene-5'-o-(Dimethoxytrityl)-5-Iodo-2'-o-Methylcytidine is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in automated oligonucleotide synthesis. This compound matters because it enables the precise, site-specific incorporation of a modified cytidine base into synthetic DNA or RNA strands, which is essential for advanced research and therapeutic development. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic laboratories working on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based therapeutics and diagnostics.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of modified DNA and RNA sequences.
• Antisense Drug Development: Incorporation into antisense oligonucleotides (ASOs) to modulate gene expression for therapeutic applications.
• siRNA and miRNA Research: Used to synthesize chemically modified small interfering RNA (siRNA) or microRNA (miRNA) to enhance stability and specificity.
• Aptamer Production: Essential for creating nuclease-resistant aptamers used in diagnostics and targeted therapeutics.
• Structural Biology Studies: Introducing specific modifications to probe nucleic acid structure, function, and protein interactions.
• Diagnostic Probe Design: Manufacturing labeled or modified probes for advanced PCR, FISH, and other molecular diagnostic techniques.

Basic Information

Product Name	N4-Diisobutylaminomethylidene-5'-o-(Dimethoxytrityl)-5-Iodo-2'-o-Methylcytidine
CAS No.	869355-36-2
Molecular Formula	C41H51IN4O6
Molecular Weight	846.79 g/mol
Synonyms	5-Iodo-2'-O-methylcytidine, N4-(Diisobutylaminomethylene) derivative, 5'-O-(4,4'-Dimethoxytrityl) ether; 5-Iodo-2'-O-methyl-5'-O-DMT-N4-(diisobutylaminomethylene)cytidine; 5-Iodo-2'-O-methylcytidine phosphoramidite precursor; DMT-5-Iodo-2'-O-Me-C(N-iBu) amidite; 869355-36-2; 5-I-2'-OMe-C Phosphoramidite
EINECS	Contact for details

Quality Control

Our N4-Diisobutylaminomethylidene-5'-o-(Dimethoxytrityl)-5-Iodo-2'-o-Methylcytidine is manufactured under strict quality systems suitable for research and pharmaceutical development. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the exacting standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with full analytical data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under an inert atmosphere (e.g., argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.