Description

N1-(4-(T-Butyl)Benzoyl)-D-(+)Biotin 2-Nitrophenyl Ester is a specialized biotin derivative designed for advanced bioconjugation and labeling applications. This compound serves as a critical reagent for the site-specific modification of biomolecules, offering a reactive ester group for efficient coupling under mild conditions. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, diagnostic assay production, and proteomics research to create targeted biotinylated probes and conjugates.

Application

• Bioconjugation Reagent: For the covalent attachment of biotin tags to proteins, antibodies, peptides, and other biomolecules.
• Diagnostic Kit Manufacturing: Used in the production of ELISA, lateral flow assays, and other immunoassays that rely on biotin-streptavidin detection systems.
• Affinity Purification Probes: Creation of biotinylated ligands for pull-down assays and the isolation of specific targets using streptavidin-coated beads.
• Chemical Biology Research: A key tool for studying protein-protein interactions, cell surface labeling, and signal transduction pathways.
• Drug Delivery Systems: Potential use in the development of targeted therapeutic conjugates where biotin acts as a targeting moiety.
• Material Science: Functionalization of surfaces and nanomaterials with biotin for biosensor and microarray applications.

Basic Information

Product Name	N1-(4-(T-Butyl)Benzoyl)-D-(+)Biotin 2-Nitrophenyl Ester
CAS No.	869354-61-0
Molecular Formula	C26H29N3O6S
Molecular Weight	511.59 g/mol
Synonyms	Biotin T-Butylbenzoyl 2-Nitrophenyl Ester; D-(+)-Biotin N1-(4-(tert-Butyl)benzoyl)-2-nitrophenyl ester; 2-Nitrophenyl N-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-4-(1,1-dimethylethyl)benzamide; Biotinylation Reagent; Active Ester of Biotin Derivative; N1-(4-tert-Butylbenzoyl)biotin 2-nitrophenyl ester
EINECS	Contact for details

Quality Control

Our N1-(4-(T-Butyl)Benzoyl)-D-(+)Biotin 2-Nitrophenyl Ester is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and structural confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in organic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.