Description

Biotin-X Nitrilotriacetic Acid, Tripotassium Salt is a high-purity, water-soluble derivative of nitrilotriacetic acid (NTA) functionalized with a biotin-X linker. This compound is specifically engineered for high-affinity bioconjugation and immobilization applications, serving as a critical reagent in advanced biochemical research and diagnostics. It is essential for researchers and manufacturers in the life sciences sector who require reliable, high-quality chelating agents for protein purification, surface functionalization, and assay development.

Application

• Protein Purification & Immobilization: Used to create biotinylated surfaces on resins, beads, or chips for the capture and study of streptavidin/avidin-tagged proteins.
• Diagnostic Assay Development: A key component in the design of ELISA, lateral flow assays, and other immunoassays requiring stable biotin-streptavidin interactions.
• Metal Chelation & Affinity Tagging: The NTA moiety allows for the reversible binding of polyhistidine-tagged (His-tag) proteins, often used in tandem with biotin for dual-affinity systems.
• Bioconjugation & Probe Synthesis: Facilitates the labeling of antibodies, enzymes, or other biomolecules with biotin for detection and imaging applications.
• Surface Plasmon Resonance (SPR): Employed to functionalize sensor chips for real-time analysis of biomolecular interactions.
• Cell Sorting & Separation: Integrated into magnetic-activated cell sorting (MACS) and other separation techniques for isolating specific cell populations.

Basic Information

Product Name	Biotin-X Nitrilotriacetic Acid, Tripotassium Salt
CAS No.	856661-92-2
Molecular Formula	C19H28KN3O8S • 2K (or similar, representing tripotassium salt)
Molecular Weight	Contact for details
Synonyms	Biotin-X NTA Tripotassium Salt; Nα-(Biotinoyl)-Nε-(carboxymethyl)-L-lysine NTA Tripotassium Salt; Biotinamidohexanoic Acid NTA Salt; Biotin-XX Nitrilotriacetic Acid Tripotassium Salt; Biotinylated Nitrilotriacetic Acid K3 Salt; Biotin-X-NTA(K3); NTA-Biotin Tripotassium Salt; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[6-[bis(carboxylatomethyl)amino]hexyl]pentanamide Tripotassium Salt
EINECS	Contact for details

Quality Control

Our Biotin-X Nitrilotriacetic Acid, Tripotassium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility suitable for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤5.0%
Solubility	Freely soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.