Description

(7-Amino-4-Methylcoumarin-3-Yl)Acetyl-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(Biotinyl)-Nh2 is a specialized, high-purity synthetic peptide substrate designed for advanced biochemical research and diagnostic applications. This compound is engineered for high sensitivity and specificity, making it a critical reagent in enzymatic activity assays and fluorescence-based detection systems. It is primarily utilized by research institutions, pharmaceutical R&D departments, and in vitro diagnostic (IVD) kit manufacturers developing assays for proteases and other key enzymes.

Application

• Protease Activity Assays: As a fluorogenic substrate for measuring the activity of specific proteolytic enzymes in research and drug discovery.
• Diagnostic Kit Development: A key component in the formulation of sensitive and specific clinical diagnostic tests for disease biomarkers.
• High-Throughput Screening (HTS): Used in automated screening platforms to identify potential therapeutic compounds that modulate enzyme activity.
• Biochemical Pathway Analysis: Enables the study of enzymatic mechanisms and signal transduction pathways in cellular models.
• IVD Manufacturing: Serves as a critical raw material for the production of commercial in vitro diagnostic reagents and kits.
• Academic & Pharmaceutical Research: Supports fundamental and applied research in enzymology, oncology, and neurology.

Basic Information

Product Name	(7-Amino-4-Methylcoumarin-3-Yl)Acetyl-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(Biotinyl)-Nh2
CAS No.	851231-19-1
Molecular Formula	C94H138N24O24S
Molecular Weight	1996.3 g/mol
Synonyms	AMC-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(Biotinyl)-NH2; Fluorogenic Peptide Substrate; Biotinylated Fluorogenic Substrate; (7-Amino-4-methylcoumarin) Peptide; Protease Substrate 851231-19-1; AMC-labeled Peptide; Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(Biotinyl)-NH2 AMC conjugate
EINECS	Contact for details

Quality Control

This high-value biochemical is manufactured under strict quality-controlled conditions to ensure batch-to-batch consistency and performance reliability. Each lot is characterized using advanced analytical techniques including HPLC for purity and Mass Spectrometry for identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, peptide content, and performance data is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.