Description

3-Azidopropanoic Acid NHS Ester is a versatile heterobifunctional crosslinking reagent featuring a reactive NHS ester and an azido group. This compound is essential for creating stable amide bonds with primary amines while introducing a bioorthogonal azide handle for subsequent click chemistry reactions, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is a critical tool for researchers and manufacturers in bioconjugation, proteomics, and materials science, enabling the precise labeling and modification of biomolecules, polymers, and surfaces.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Used to site-specifically conjugate cytotoxic drugs, fluorophores, or other payloads to antibodies, proteins, and peptides.
• Proteomics and Biomarker Discovery: Facilitates the labeling and enrichment of proteins for mass spectrometry analysis and activity-based protein profiling (ABPP).
• Polymer and Materials Functionalization: Enables the surface modification of nanoparticles, hydrogels, and other polymeric materials with bioactive ligands.
• Chemical Biology Probe Synthesis: Serves as a key building block for creating activity-based probes to study enzyme function and metabolic pathways.
• Diagnostic Reagent Manufacturing: Incorporated into the production of diagnostic assays, such as ELISA kits, for detecting specific antigens or antibodies.
• Glycan and Carbohydrate Labeling: Used to tag complex sugars for imaging, purification, and interaction studies.

Basic Information

Product Name	3-Azidopropanoic Acid NHS Ester
CAS No.	850180-76-6
Molecular Formula	C9H10N4O5
Molecular Weight	254.20 g/mol
Synonyms	N-(3-Azidopropionyloxy)succinimide; 3-Azidopropionic Acid N-Hydroxysuccinimide Ester; NHS-Azide; Azido-PEG1-NHS Ester; N-Succinimidyl 3-Azidopropanoate; Azidopropanoic Acid Succinimidyl Ester; β-Azidopropionic Acid NHS Ester; 3-Azido-1-propanoic Acid 2,5-Dioxo-1-pyrrolidinyl Ester
EINECS	Contact for details

Quality Control

Our 3-Azidopropanoic Acid NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and FT-IR, to ensure high purity and consistent performance. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.