Description

4-Methylumbelliferyl 2-Acetamido-2-Deoxy-3,6-Dipivaloyl--D-Galactopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of specific glycosidase enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise enzymatic assays in diagnostic and biochemical research. It is primarily utilized in the life sciences sector for applications ranging from high-throughput screening to the study of carbohydrate-active enzymes.

Application

• Enzymatic Assay Substrate: A key fluorogenic reagent for detecting and measuring the activity of β-N-acetylhexosaminidases and related glycosidases.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of enzyme specificity within carbohydrate metabolism pathways.
• Diagnostic Development: Employed in the research and development of diagnostic kits for lysosomal storage disorders, such as Tay-Sachs and Sandhoff diseases.
• High-Throughput Screening (HTS): Ideal for automated screening platforms in pharmaceutical discovery to identify potential therapeutic enzyme modulators.
• Microbiology: Can be applied in assays to study bacterial glycosidases for microbiological identification and research.
• Quality Control: Serves as a reference standard in the quality control of enzyme preparations and diagnostic reagents.

Basic Information

Product Name	4-Methylumbelliferyl 2-Acetamido-2-Deoxy-3,6-Dipivaloyl--D-Galactopyranoside
CAS No.	849207-59-6
Molecular Formula	C29H39NO12
Molecular Weight	593.62 g/mol
Synonyms	4-MU-β-D-GalNAc(3,6-diPiv); 4-Methylumbelliferyl N-acetyl-β-D-galactosaminide (3,6-di-pivaloyl); 4-MU-β-GalNAc(3,6-diPiv); 4-Methylumbelliferyl 2-acetamido-2-deoxy-3,6-di-O-pivaloyl-β-D-galactopyranoside; Fluorogenic GalNAc substrate; 4-MU-GalNAc(3,6-diPiv); β-N-Acetylhexosaminidase fluorogenic substrate; 849207-59-6
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl 2-Acetamido-2-Deoxy-3,6-Dipivaloyl--D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available for every lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol
Fluorogenic Activity	Functional in standard enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.