Description

2-Fluoro-4-Nitrophenyl B-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate designed for the sensitive detection of β-galactosidase enzyme activity. This compound matters for researchers and industrial developers who require precise, high-throughput enzymatic assays, particularly in molecular biology, diagnostics, and high-content screening applications. It is primarily needed by biotechnology firms, pharmaceutical R&D laboratories, and diagnostic kit manufacturers for applications in reporter gene assays, cell-based screening, and microbiological testing.

Application

• Reporter Gene Assays: A key substrate for detecting β-galactosidase expression in molecular biology and genetic engineering.
• Diagnostic Development: Used in the formulation of clinical and research diagnostic kits for enzyme-linked detection.
• High-Throughput Screening (HTS): Enables cell-based and biochemical screening for drug discovery and functional genomics.
• Microbiology Research: Employed in studies of bacterial gene expression (e.g., lacZ gene) and microbial identification.
• Enzyme Kinetics & Characterization: Serves as a specific substrate for studying the kinetics and inhibition of β-galactosidase enzymes.
• Histochemistry & Cell Staining: Used in techniques for visualizing β-galactosidase activity in tissues and fixed cells.

Basic Information

Product Name	2-Fluoro-4-Nitrophenyl B-D-Galactopyranoside
CAS No.	807610-60-2
Molecular Formula	C12H14FNO8
Molecular Weight	319.24 g/mol
Synonyms	2-Fluoro-4-nitrophenyl β-D-galactopyranoside; 4-Nitro-2-fluorophenyl β-D-galactopyranoside; β-D-Galactopyranoside, 2-fluoro-4-nitrophenyl; FNP-Gal; 2-F-4-NPG; Galactopyranoside derivative, fluoronitrophenyl; Enzymatic substrate for β-galactosidase; Chromogenic β-galactosidase substrate.
EINECS	Contact for details

Quality Control

Every batch of 2-Fluoro-4-Nitrophenyl B-D-Galactopyranoside is manufactured and tested under a strict quality management system. We guarantee high-purity material suitable for sensitive research and diagnostic applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed HPLC purity data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light. For long-term storage, consider desiccants and inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, colorless solution in DMSO or DMF
Specific Rotation	Contact for details
Biological Activity	Functional as β-galactosidase substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.