Description

Benzyloxycarbonyl Glycyl-Glycyl-Arginine-4-Nitroanilide is a synthetic, chromogenic peptide substrate designed for the specific detection and quantification of proteolytic enzyme activity. This compound matters because it provides a highly sensitive and reliable method for enzymatic assays, particularly in research and diagnostic applications. It is primarily needed by biochemists, pharmaceutical researchers, and diagnostic kit manufacturers working in enzymology, protease inhibitor screening, and clinical diagnostics.

Application

• Protease Activity Assays: A key chromogenic substrate for measuring the activity of trypsin-like serine proteases.
• Biochemical Research: Used in kinetic studies and enzyme characterization in academic and industrial R&D laboratories.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and evaluate novel protease inhibitors as potential drug candidates.
• Diagnostic Reagents: A critical component in the formulation of diagnostic kits for conditions related to abnormal protease activity.
• Quality Control: Used in biomanufacturing to monitor and validate the purity and activity of enzyme preparations.

Basic Information

Product Name	Benzyloxycarbonyl Glycyl-Glycyl-Arginine-4-Nitroanilide
CAS No.	89991-62-8
Molecular Formula	C24H31N9O7
Molecular Weight	557.56 g/mol
Synonyms	Z-Gly-Gly-Arg-pNA; Z-Gly-Gly-Arg-4-nitroanilide; Cbz-Gly-Gly-Arg-pNA; Benzyloxycarbonyl-Gly-Gly-Arg-4-nitroanilide; Carbobenzoxy-Gly-Gly-Arg-pNA; Chromogenic Substrate for Trypsin; Nα-CBZ-Glycyl-glycyl-L-arginine 4-nitroanilide acetate salt; 89991-62-8
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonyl Glycyl-Glycyl-Arginine-4-Nitroanilide is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive enzymatic assays. All products undergo rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with each shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccation. Allow the product to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.