Description

Biotin-Xx-Nhs CAS NO 89889-52-1 is a high-purity, amine-reactive biotinylation reagent designed for the stable conjugation of biomolecules. This compound is critical for researchers and manufacturers requiring precise and efficient labeling of proteins, antibodies, and other amine-containing targets for detection, purification, and diagnostic applications. It is an essential tool for professionals in bioconjugation, assay development, and diagnostic kit manufacturing, enabling the creation of highly sensitive and specific detection systems.

Application

• Bioconjugation and Protein Labeling: Primary reagent for covalently attaching biotin to primary amines on antibodies, enzymes, and other proteins.
• Immunoassay Development: Used to create biotinylated detection antibodies and antigens for ELISA, lateral flow assays, and other immunoassay formats.
• Diagnostic Kit Manufacturing: Essential component in the production of commercial diagnostic tests that utilize streptavidin-biotin technology for signal amplification.
• Affinity Purification Systems: Enables the biotinylation of ligands for capture and purification using streptavidin or avidin resins.
• Cell Surface Labeling: Facilitates the tagging of cell surface proteins for flow cytometry, microscopy, and other cell-based analyses.
• Microarray and Biosensor Fabrication: Used to functionalize surfaces with biotin for the immobilization of biomolecules in research and diagnostic platforms.
• Nucleic Acid Probe Labeling: Applicable for modifying amine-modified oligonucleotides for hybridization-based detection methods.

Basic Information

Product Name	Biotin-Xx-Nhs
CAS No.	89889-52-1
Molecular Formula	C26H41N5O8S
Molecular Weight	583.70 g/mol
Synonyms	Biotin-XX, SSE; Biotinamidocaproylamidohexanoic acid N-hydroxysuccinimide ester; Biotin-XX-NHS ester; Biotin-LC-LC-NHS; (+)-Biotinyl-6-aminocaproyl-6-aminocaproic acid N-succinimidyl ester; N-Succinimidyl 6-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)amino)hexanoate; Biotin (long chain) NHS ester
EINECS	Contact for details

Quality Control

Our Biotin-Xx-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment to guarantee traceability, consistency, and performance.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.