Description

4-Nitrophenyl-β-D-Glucuronic Acid, Sodium Salt is a chromogenic substrate widely used in enzymatic assays. It serves as a critical reagent for the detection and quantification of β-glucuronidase activity, an important biomarker and diagnostic tool. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries.

Application

• Enzymatic Assay Substrate: Primary substrate for the spectrophotometric determination of β-glucuronidase enzyme activity in research and clinical diagnostics.
• Diagnostic Kits: Key component in commercial diagnostic kits for detecting bacterial contamination (e.g., E. coli) and certain genetic disorders.
• Pharmaceutical Research: Used in high-throughput screening (HTS) to identify potential inhibitors or activators of the β-glucuronidase enzyme for drug discovery.
• Microbiology: Employed in microbiological media and tests for the specific identification of bacteria expressing β-glucuronidase.
• Biochemical Research: Tool for studying enzyme kinetics, reaction mechanisms, and glycoside hydrolysis in biochemical pathways.
• Quality Control: Used as a reference standard in the quality control of enzymes and diagnostic reagents.

Basic Information

Product Name	4-Nitrophenyl-β-D-Glucuronic Acid, Sodium Salt
CAS No.	89772-41-8
Molecular Formula	C12H12NNaO10
Molecular Weight	353.21 g/mol
Synonyms	p-Nitrophenyl β-D-Glucuronide Sodium Salt; 4-Nitrophenyl β-D-Glucopyranosiduronic Acid Sodium Salt; PNPG Sodium Salt; pNPG Sodium Salt; β-Glucuronidase Substrate; 4-NPG Sodium Salt; 1-(4-Nitrophenyl)-β-D-glucopyranosiduronic Acid Sodium Salt
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-β-D-Glucuronic Acid, Sodium Salt is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive analytical and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in enzymatic assays. We adhere to relevant quality guidelines to support our customers in research, pharmaceutical development, and in-vitro diagnostic (IVD) manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Absorbance (Lambda Max)	Conforms
Enzymatic Activity	Suitable for β-glucuronidase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.