Description

n-α-Cbz-Arg-Arg 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound matters as a critical reagent for the continuous, real-time monitoring of protease activity, particularly for trypsin-like enzymes such as furin and other proprotein convertases. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors who require precise tools for studying enzyme kinetics, inhibitor screening, and diagnostic assay development.

Application

• Protease Activity Assays: Primary substrate for fluorometrically measuring the activity of trypsin, furin, and related serine proteases.
• Enzyme Kinetics & Inhibitor Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Biochemical Research: A key tool for studying the enzymatic cleavage mechanisms and specificity of arginine-specific proteases.
• Diagnostic Reagent Development: Employed in the development of fluorogenic assays for clinical diagnostics and pathogen detection.
• Cell Biology Studies: Used to monitor protease activity in cellular and tissue extracts.
• Quality Control in Biologics Manufacturing: Applied in purity and potency testing of therapeutic enzymes and related products.

Basic Information

Product Name	n-α-Cbz-Arg-Arg 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	88937-61-5
Molecular Formula	C34H47N11O8 • HCl
Molecular Weight	774.23 g/mol (Free base: 737.81 g/mol)
Synonyms	Z-Arg-Arg-AMC • HCl; Cbz-Arg-Arg-7-amido-4-methylcoumarin hydrochloride; Benzyloxycarbonyl-Arg-Arg-AMC • HCl; Nα-Carbobenzoxy-L-arginyl-L-arginine 4-methyl-7-coumarylamide hydrochloride; Furin Substrate; Boc-Arg-Val-Arg-Arg-MCA (related class); Fluorogenic Protease Substrate 88937-61-5; Trypsin Substrate Z-Arg-Arg-AMC
EINECS	Contact for details

Quality Control

Our n-α-Cbz-Arg-Arg 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure batch-to-batch consistency and high performance in sensitive fluorometric applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to stringent quality management practices suitable for research and development use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spec)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Fluorogenic Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.