Description

Cholesterylsuccinyl-n-Hydroxysuccinimide is a high-purity, activated ester derivative of cholesterol, specifically designed for bioconjugation and labeling applications. This compound matters because it provides a reactive handle for covalently attaching cholesterol moieties to amines on peptides, proteins, or other biomolecules, thereby modifying their properties for research and development. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors working on drug delivery systems, lipid nanoparticle (LNP) formulation, and membrane protein studies.

Application

• Bioconjugation Reagent: Used for the covalent attachment of cholesterol to peptides, proteins, antibodies, or oligonucleotides to enhance membrane association or stability.
• Lipid Nanoparticle (LNP) Research: Serves as a functionalized lipid component in the development and optimization of LNPs for mRNA, siRNA, and other nucleic acid delivery platforms.
• Membrane Biology Studies: Employed to label or modify membrane proteins and lipids to study cellular uptake, trafficking, and localization.
• Drug Delivery System Development: Acts as a key synthetic intermediate for creating cholesterol-anchored prodrugs or targeted therapeutic carriers.
• Diagnostic Reagent Synthesis: Utilized in the preparation of cholesterol-labeled probes and contrast agents for imaging and diagnostic assays.
• Biomaterial Functionalization: Applied to introduce hydrophobic cholesterol anchors onto surfaces or polymers to create biomimetic materials.

Basic Information

Product Name	Cholesterylsuccinyl-n-Hydroxysuccinimide
CAS No.	88848-79-7
Molecular Formula	C35H55NO5
Molecular Weight	569.82 g/mol
Synonyms	Cholesteryl Hemisuccinate N-Hydroxysuccinimide Ester; Cholesterol NHS Ester; NSC 334368; Cholest-5-en-3β-yl 3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropanoate; 3β-[(3-Carboxy-1-oxopropoxy)]cholest-5-en-3β-yl N-succinimidyl ester; Cholesterylsuccinyl NHS ester; Cholesterol-hemisuccinate-NHS; CS-NHS
EINECS	Contact for details

Quality Control

Our Cholesterylsuccinyl-n-Hydroxysuccinimide is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for research and bioconjugation applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic identification. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, chloroform; slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.