Description

H-Norleucyl-Hexahydrotyrosyl-Lysine-4-Nitroanilide is a specialized synthetic peptide substrate designed for enzymatic research and diagnostic applications. This compound is critical for the precise measurement of protease and peptidase activity, particularly in biochemical assays and pharmaceutical development. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics studies and high-throughput screening.

Application

• Protease/Peptidase Activity Assays: A key chromogenic substrate for quantifying the activity of specific serine and cysteine proteases in research settings.
• Diagnostic Kit Manufacturing: Used as a critical component in the formulation of clinical diagnostic kits for detecting enzyme-related pathologies.
• Pharmaceutical R&D: Employed in high-throughput screening (HTS) platforms to identify and characterize new enzyme inhibitors for drug discovery.
• Biochemical Research: Serves as a tool for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial laboratories.
• Quality Control in Biologics: Applied in the purity and potency testing of enzyme-based therapeutic products during manufacturing.

Basic Information

Product Name	H-Norleucyl-Hexahydrotyrosyl-Lysine-4-Nitroanilide
CAS No.	88793-96-8
Molecular Formula	C30H50N6O7
Molecular Weight	606.77 g/mol
Synonyms	H-Nle-Hht-Lys-pNA; Norleucyl-Hexahydrotyrosyl-Lysine-pNA; Substrate for Protease; Chromogenic Peptide Substrate; Lys-p-Nitroanilide Derivative; 4-Nitroanilide Peptide Substrate; Protease Substrate 88793-96-8; Nle-Hht-Lys-pNA
EINECS	Contact for details

Quality Control

Our H-Norleucyl-Hexahydrotyrosyl-Lysine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for functional performance. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided to ensure compliance with research and diagnostic-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Extinction Coefficient	Provided on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.