Description

n-p-Tosyl-Gly-Pro-Lys p-Nitroanilide Acetate Salt is a specialized chromogenic peptide substrate designed for enzymatic activity assays. This compound is critical for researchers and quality control laboratories requiring precise, colorimetric detection of specific protease activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and biochemical research.

Application

• Protease Activity Assays: Used as a specific chromogenic substrate for the detection and quantification of trypsin, plasmin, and related serine proteases.
• Biochemical Research: Essential tool for studying enzyme kinetics, mechanism of action, and inhibition profiles in academic and industrial R&D settings.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic protease inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical analysis of blood coagulation and fibrinolysis pathways.
• Quality Control Testing: Used to standardize and validate the activity of enzyme preparations in biopharmaceutical production.

Basic Information

Product Name	n-p-Tosyl-Gly-Pro-Lys p-Nitroanilide Acetate Salt
CAS No.	88793-79-7
Molecular Formula	C30H40N8O9S • xC2H4O2
Molecular Weight	664.76 g/mol (Free base)
Synonyms	Tosyl-Gly-Pro-Lys-pNA Acetate Salt; Tos-Gly-Pro-Lys-pNA • AcOH; Chromogenic Substrate S-2251 (H-D-Val-Leu-Lys-pNA analog); H-D-Val-Leu-Lys-pNA Substrate Analog; N-p-Tosyl-Glycyl-L-prolyl-L-lysine 4-nitroanilide acetate; Glycyl-prolyl-lysine p-nitroanilide tosylate salt; Protease Substrate 88793-79-7
EINECS	Contact for details

Quality Control

Our n-p-Tosyl-Gly-Pro-Lys p-Nitroanilide Acetate Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing assay, impurity profile, and physical characteristics are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or moisture will affect stability and performance. For long-term storage, consider desiccant and under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Absorbance Spectrum	Conforms to reference (λmax ~315 nm, ~405 nm for p-nitroaniline)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.