Description

N(Alpha)-Acetyl-N(Epsilon)-4-(7-Nitrobenzofurazanyl)Lysyl-Alanyl-Alanine is a specialized, custom-designed peptide derivative that incorporates a fluorescent nitrobenzofurazan (NBD) reporter group. This compound is engineered for advanced biochemical research and diagnostic applications, offering a unique tool for labeling, detection, and mechanistic studies. It is primarily utilized by researchers and developers in the fields of proteomics, enzymology, and the development of novel diagnostic assays.

Application

• Fluorescent Labeling Agent: Used as a covalent label for proteins, peptides, and other biomolecules to enable fluorescence-based detection and tracking.
• Protease Substrate & Assay Development: Serves as a specific, sensitive substrate for studying protease activity, enzyme kinetics, and inhibitor screening in high-throughput assays.
• Biochemical Research Probe: Acts as a critical tool in mechanistic studies of protein-protein interactions, signal transduction pathways, and cellular localization.
• Diagnostic Reagent Component: Incorporated into the development of advanced in-vitro diagnostic (IVD) kits and biosensors for clinical and research laboratories.
• Peptide Chemistry & Conjugation: Employed in synthetic peptide chemistry for introducing a fluorescent tag into custom peptide sequences.

Basic Information

Product Name	N(Alpha)-Acetyl-N(Epsilon)-4-(7-Nitrobenzofurazanyl)Lysyl-Alanyl-Alanine
CAS No.	88721-92-0
Molecular Formula	C24H32N8O9
Molecular Weight	576.56 g/mol
Synonyms	Ac-Lys(NBD)-Ala-Ala-OH; Nα-Acetyl-Nε-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-L-lysyl-L-alanyl-L-alanine; NBD-Lys(Ac)-Ala-Ala; NBD-labeled peptide substrate; 4-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl))-Nα-acetyl-L-lysyl-L-alanyl-L-alanine; Fluorescent peptide derivative; NBD-X-Ac-Lys-Ala-Ala
EINECS	Contact for details

Quality Control

Our production of N(Alpha)-Acetyl-N(Epsilon)-4-(7-Nitrobenzofurazanyl)Lysyl-Alanyl-Alanine adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is characterized using advanced analytical techniques including HPLC, MS, and NMR to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, keep desiccated. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 97% (on dry basis)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.