Description

Chymotrypsin Substrate Ii, Fluorogenic is a specialized synthetic peptide derivative designed for the sensitive and continuous detection of chymotrypsin activity. This compound matters as a critical tool for researchers and quality control labs requiring precise enzymatic analysis, offering high sensitivity and specificity in fluorometric assays. It is essential for professionals in pharmaceutical R&D, diagnostic kit manufacturing, and biochemical research focused on protease function and inhibitor screening.

Application

• Biochemical Research: Used as a selective fluorogenic substrate for kinetic studies and characterization of chymotrypsin and related serine proteases.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) assays to identify and evaluate potential chymotrypsin inhibitors for therapeutic applications.
• Diagnostic Manufacturing: Serves as a key component in the formulation of fluorogenic assay kits for clinical and research diagnostics targeting protease activity.
• Quality Control (QC): Applied in biopharmaceutical QC labs to monitor the activity and consistency of enzyme preparations and related bioprocesses.
• Cell Biology & Proteomics: Facilitates the study of protease roles in cellular pathways and protein degradation processes within complex biological samples.

Basic Information

Product Name	Chymotrypsin Substrate Ii, Fluorogenic
CAS No.	88467-45-2
Molecular Formula	C34H37N5O6
Molecular Weight	611.69 g/mol
Synonyms	Suc-Ala-Ala-Pro-Phe-AMC; N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin; Suc-AAPF-AMC; Fluorogenic Chymotrypsin Substrate; Chymotrypsin Substrate Suc-AAPF-AMC; AMC-labeled Chymotrypsin Substrate; 7-Amido-4-methylcoumarin Chymotrypsin Substrate; Protease Fluorogenic Substrate I
EINECS	Contact for details

Quality Control

Our Chymotrypsin Substrate Ii, Fluorogenic is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and spectrophotometric verification, to ensure it meets high-purity research-grade specifications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.