Description

Suc-Ala-Pro-Ala-Amc CAS NO 88467-44-1 is a fluorogenic peptide substrate specifically designed for the detection and quantification of protease activity. This compound is a critical tool in biochemical research, enabling precise measurement of enzymatic kinetics and inhibitor screening. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors for studying proteases like dipeptidyl peptidase and related enzymes.

Application

• Protease Activity Assays: As a fluorogenic substrate for kinetic studies of dipeptidyl peptidase IV (DPP-IV) and related proteases.
• Drug Discovery & Development: High-throughput screening (HTS) for identifying and characterizing novel protease inhibitors.
• Biochemical Research: Fundamental research into protease specificity, mechanism of action, and enzymatic pathways.
• Diagnostic Reagent Development: A key component in developing fluorometric diagnostic kits for clinical and research use.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of biopharmaceuticals.
• Enzyme Characterization: Determining kinetic parameters (Km, Vmax, kcat) for purified enzyme preparations.

Basic Information

Product Name	Suc-Ala-Pro-Ala-Amc
CAS No.	88467-44-1
Molecular Formula	C23H30N6O7
Molecular Weight	502.52 g/mol
Synonyms	Suc-Ala-Pro-Ala-AMC; Suc-APA-AMC; Sucinyl-Ala-Pro-Ala-7-amido-4-methylcoumarin; N-Succinyl-Ala-Pro-Ala-7-amido-4-methylcoumarin; Substrate for DPP-IV; DPP-IV Substrate Suc-APA-AMC; Fluorogenic Peptide Substrate Suc-APA-AMC; N-Succinyl-Alanyl-Prolyl-Alanyl-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Suc-Ala-Pro-Ala-Amc is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity verification, to ensure it meets the high standards required for sensitive research applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming specifications for purity, appearance, and performance in assay systems.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF
Functional Activity	Suitable for fluorometric assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.