Description

4-Azido-2-Nitrophenyluridylyl Pyrophosphate is a specialized nucleotide analog designed for advanced biochemical research and development. This compound is critical for applications requiring photoactivatable cross-linking or affinity labeling, enabling precise molecular targeting and analysis. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, enzymology, and pharmaceutical development for studying nucleic acid-protein interactions and developing novel diagnostic tools.

Application

• Photoaffinity Labeling: Acts as a key reagent for cross-linking studies to identify and characterize protein binding sites on RNA or DNA.
• Enzyme Mechanism Studies: Used as a substrate or inhibitor analog to probe the active sites and catalytic mechanisms of polymerases, kinases, and other nucleotide-processing enzymes.
• Probe Development: Serves as a building block for synthesizing labeled nucleotides used in advanced detection and diagnostic assays.
• Structural Biology: Aids in the structural analysis of nucleic acid complexes through cross-linking experiments followed by mass spectrometry or crystallography.
• Drug Discovery Research: Employed in high-throughput screening to identify compounds that interact with specific nucleotide-binding domains.
• Bioconjugation: Facilitates the site-specific attachment of biomolecules to surfaces or other molecules for biosensor and microarray development.

Basic Information

Product Name	4-Azido-2-Nitrophenyluridylyl Pyrophosphate
CAS No.	88159-20-0
Molecular Formula	C16H17N6O17P3
Molecular Weight	658.24 g/mol
Synonyms	4-Azido-2-nitrophenyl-uridylyl pyrophosphate; 4-Azido-2-nitrophenyl-UDP; ANP-UDP; 4-Azido-2-nitrophenyl uridine diphosphate; 4-Azido-2-nitrophenyluridine 5'-diphosphate; 4-Azido-2-nitrophenyl-5'-uridylyl diphosphate; Photoactivatable UDP analog; 2'-Deoxy-? (Contact for specific isomer details)
EINECS	Contact for details

Quality Control

Our 4-Azido-2-Nitrophenyluridylyl Pyrophosphate is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and structural confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is strictly light-sensitive and should be handled under subdued lighting conditions. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation. Store away from strong oxidizers and reducing agents.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.