Description

n-Acetyl-L-Glutamine p-Nitroanilide is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the study and detection of specific proteolytic enzymes, offering high sensitivity and selectivity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics, inhibitor screening, and diagnostic development.

Application

• Enzymatic Substrate for Protease Research: Used as a specific chromogenic substrate for studying the activity of proteases like glutamyl endopeptidases.
• Biochemical Assay Development: Essential for developing colorimetric and spectrophotometric assays to measure enzyme kinetics and inhibition.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical and research laboratories.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for drug discovery.
• Academic & Industrial Research: Supports fundamental biochemical research in universities, government labs, and biotech R&D departments.
• Quality Control in Biologics: Can be used in QC testing for processes involving specific enzymatic activities.

Basic Information

Product Name	n-Acetyl-L-Glutamine p-Nitroanilide
CAS No.	87811-40-3
Molecular Formula	C₁₃H₁₆N₄O₆
Molecular Weight	324.29 g/mol
Synonyms	N-Acetyl-L-glutamine 4-nitroanilide; Ac-Gln-pNA; L-Glutamine, N-acetyl-, 4-nitroanilide; N-Acetyl-L-glutamine p-nitroanilide; Nα-Acetyl-L-glutamine p-nitroanilide; Acetylglutamine p-nitroanilide; Substrate for Glutamyl Endopeptidase; 87811-40-3
EINECS	Contact for details

Quality Control

Our n-Acetyl-L-Glutamine p-Nitroanilide is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. We provide comprehensive Certificates of Analysis (COA) with each batch, detailing purity, identity, and impurity profiles. Our quality commitment aligns with the standards expected by the pharmaceutical and diagnostic industries.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.