Description

4-[3-(Trifluoromethyl)Diazirin-3-Yl]Benzoic Acid n-Hydroxysuccinimide Ester is a highly specialized photoactivatable crosslinking reagent. This compound is critical for advanced biochemical research, enabling the covalent labeling and capture of transient biomolecular interactions. It is primarily utilized by researchers in pharmaceutical R&D, proteomics, and structural biology for mapping protein-protein interactions and identifying binding sites.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalent crosslinking in photoaffinity labeling experiments to study ligand-receptor or protein-protein interactions.
• Chemical Proteomics: Used to create activity-based probes (ABPs) for profiling enzyme families and identifying drug targets in complex biological samples.
• Bioconjugation: Facilitates the stable attachment of small molecules, peptides, or reporter tags (e.g., fluorophores, biotin) to proteins and other biomolecules via its NHS ester group.
• Structural Biology: Employed to introduce crosslinks for analyzing protein structure and dynamics using techniques like mass spectrometry.
• Drug Discovery: Integral to identifying off-targets and mapping the binding sites of novel therapeutic compounds.
• Material Science: Used to functionalize surfaces and polymers with bioactive molecules for biosensor and diagnostic device development.

Basic Information

Product Name	4-[3-(Trifluoromethyl)Diazirin-3-Yl]Benzoic Acid n-Hydroxysuccinimide Ester
CAS No.	87736-89-8
Molecular Formula	C15H9F3N3O4
Molecular Weight	352.25 g/mol
Synonyms	3-(3-(Trifluoromethyl)-3H-diazirin-3-yl)benzoic acid N-hydroxysuccinimide ester; 4-(3-(Trifluoromethyl)diazirin-3-yl)benzoic acid NHS ester; TFD-NHS Ester; 3-(3-(Trifluoromethyl)diazirin-3-yl)benzoyloxysuccinimide; Benzophenone-4-(3-(trifluoromethyl)-3H-diazirin-3-yl)benzoic acid NHS ester analog; Photoactivatable NHS ester; Photo-leucine analog crosslinker; PAL Crosslinker, Trifluoromethyl Diazirine NHS Ester
EINECS	Contact for details

Quality Control

Our 4-[3-(Trifluoromethyl)Diazirin-3-Yl]Benzoic Acid n-Hydroxysuccinimide Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.