Description

D-Threonine Aldolase (Crude Enzyme) is a biocatalyst that facilitates the reversible aldol cleavage of D-threonine into glycine and acetaldehyde. This enzyme is critical for the stereoselective synthesis of β-hydroxy-α-amino acids, which are valuable chiral building blocks in pharmaceutical and fine chemical manufacturing. It is an essential tool for research and production in the fields of asymmetric synthesis, biocatalysis, and the development of novel APIs and intermediates.

Application

• Asymmetric Synthesis of β-Hydroxy-α-Amino Acids: Key catalyst for the stereoselective production of non-natural amino acids used in drug discovery.
• Pharmaceutical Intermediate Manufacturing: Used in the biocatalytic synthesis of chiral intermediates for antibiotics, protease inhibitors, and other active pharmaceutical ingredients (APIs).
• Biocatalysis Process Development: Employed in R&D for developing efficient, green chemistry routes to replace traditional chemical synthesis methods.
• Enzymatic Resolution: Facilitates the kinetic resolution of racemic mixtures to obtain enantiomerically pure compounds.
• Biochemical Research: Used as a model enzyme in studies of aldolase mechanisms, enzyme kinetics, and metabolic pathway engineering.
• Fine Chemical Production: Applied in the manufacture of specialty chemicals requiring high stereochemical purity.

Basic Information

Product Name	D-Threonine Aldolase (Crude Enzyme)
CAS No.	87588-22-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	D-Threonine Aldolase; DTA; D-Threonine Aldolase (crude); EC 4.1.2.48; (R)-Threonine Aldolase; D-Threonine Acetaldehyde-Lyase; Glycine Acetaldehyde-Lyase; Aldolase T
EINECS	Contact for details

Quality Control

Our D-Threonine Aldolase (Crude Enzyme) is produced and tested under a rigorous quality management system. Each batch is controlled for key activity parameters and purity to ensure consistent performance in biocatalytic applications. While specific activity and protein content can vary by batch, comprehensive Certificates of Analysis (COA) detailing enzyme activity (U/mg), protein concentration, and buffer composition are provided to support your process development and quality assurance.

Storage

Preserve in a tightly closed container, protected from light. For crude enzyme preparations, store at 2-8°C for short-term stability. For long-term storage, aliquoting and freezing at -20°C or below is recommended. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent moisture absorption and loss of activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Catalyzes D-threonine aldol cleavage
Protein Content (Bradford)	Specified per batch on COA
Specific Activity	≥ [Value] U/mg protein (Batch-specific)
pH Optimum	~7.5 - 8.5
Buffer System	Typically phosphate or Tris buffer
Stabilizers/Additives	May contain glycerol, DTT, or BSA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.