Description

p-Nitrophenyl 6-Fluoro-6-Deoxy-B-D-Glucopyranoside is a specialized fluorinated glycoside derivative, serving as a critical chromogenic substrate for enzymatic assays. Its core value lies in enabling precise, colorimetric detection and quantification of specific glycosidase enzyme activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable tools for enzyme kinetics, inhibitor screening, and biochemical pathway analysis.

Application

• Enzyme Substrate for Glycosidases: Primary use as a chromogenic substrate for β-glucosidase and related hydrolytic enzymes, where cleavage releases the yellow p-nitrophenol for easy spectrophotometric detection.
• Biochemical Research & Assay Development: A key reagent in high-throughput screening (HTS) platforms for identifying and characterizing enzyme inhibitors or activators in drug discovery.
• Diagnostic Kit Manufacturing: Used in the formulation of clinical diagnostic kits for measuring specific enzyme levels, aiding in the detection of genetic disorders like Gaucher disease.
• Microbiology & Cell Biology Studies: Employed to study glycosidase activity in various microorganisms and cell lysates, providing insights into metabolic functions and cellular processes.
• Quality Control in Bioprocessing: Monitors enzyme activity and purity during the production and purification of therapeutic proteins and recombinant enzymes.

Basic Information

Product Name	p-Nitrophenyl 6-Fluoro-6-Deoxy-B-D-Glucopyranoside
CAS No.	87585-95-3
Molecular Formula	C12H14FNO7
Molecular Weight	303.24 g/mol
Synonyms	6-Fluoro-6-deoxy-4-nitrophenyl β-D-glucopyranoside; p-Nitrophenyl 6-fluoro-6-deoxy-β-D-glucoside; 4-Nitrophenyl 6-fluoro-6-deoxy-β-D-glucopyranoside; 6-Fluoro-4-nitrophenyl β-D-glucopyranoside; PNPG-6F; 6-Fluoro-PNPG; β-D-Glucopyranoside, 6-fluoro-6-deoxy-, 4-nitrophenyl
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 6-Fluoro-6-Deoxy-B-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	95.0 - 105.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.