Description

Malonylbis(Methionyl-4-Nitrophenyl Ester) is a specialized biochemical reagent primarily used as a substrate or building block in enzymatic and synthetic research. This compound matters for its role in studying protease and peptidase activity, as the 4-nitrophenyl ester group is a classic chromogenic leaving group. It is needed by research scientists and process developers in the pharmaceutical, biotechnology, and life science sectors for applications in enzyme kinetics, inhibitor screening, and peptide synthesis.

Application

• Enzyme Substrate: Used as a chromogenic substrate for the study of various proteases and esterases, where cleavage releases 4-nitrophenol for spectrophotometric detection.
• Biochemical Research: Serves as a key reagent in fundamental research on enzyme mechanisms, kinetics, and inhibition studies.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) assays to identify and characterize potential therapeutic enzyme inhibitors.
• Peptide Synthesis Intermediate: Acts as an activated ester derivative in solid-phase and solution-phase peptide synthesis, particularly for incorporating methionine or malonyl-based structures.
• Diagnostic Reagent Manufacturing: Utilized in the formulation of diagnostic kits and reagents for clinical and research laboratories.

Basic Information

Product Name	Malonylbis(Methionyl-4-Nitrophenyl Ester)
CAS No.	87498-80-4
Molecular Formula	C23H28N4O10S2
Molecular Weight	608.62 g/mol
Synonyms	Bis(4-nitrophenyl) 3,3'-((methionyl)azanediyl)dipropanoate; Malonyl-bis-L-methionine 4-nitrophenyl ester; Malonylbis(methionyl-4-nitrophenyl ester); L-Methionine, N-[3-[[[1-(4-nitrophenyl)-1,3-dioxopropyl]amino]carbonyl]propionyl]-, 4-nitrophenyl ester; Substrate for proteolytic enzymes; Activated malonyl-methionine ester; p-Nitrophenyl malonylbis(methioninate)
EINECS	Contact for details

Quality Control

Our Malonylbis(Methionyl-4-Nitrophenyl Ester) is produced under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by spectroscopic methods. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.