Description

1-(3'-C-Methyl-β-D-Ribofuranosyl)Uracil 5'-Triphosphate is a modified nucleoside triphosphate analog, a critical building block for advanced biochemical research and development. Its primary value lies in its ability to be incorporated into nucleic acid polymers, enabling the study of RNA structure, function, and stability, particularly in the context of modified nucleotides. This high-purity biochemical is essential for researchers and developers in pharmaceutical discovery, molecular diagnostics, and enzymology. It serves as a key reagent for synthesizing modified RNA oligonucleotides used in therapeutic applications and fundamental biological studies.

Application

• RNA Synthesis & Therapeutics: As a modified substrate for in vitro transcription (IVT) and enzymatic synthesis of therapeutic RNA, including mRNA vaccines and siRNA.
• Structural Biology Studies: Incorporation into RNA oligonucleotides to investigate the impact of 2'-C-methyl modifications on RNA folding, stability, and protein-RNA interactions.
• Enzyme Mechanism & Kinetics: Used as a specific substrate to study the activity, fidelity, and inhibition of viral RNA-dependent RNA polymerases (RdRps), such as those from HCV and other viruses.
• Diagnostic Probe Development: Enables the creation of high-affinity, nuclease-resistant nucleic acid probes for detection assays.
• Antiviral Drug Discovery: Acts as a chain terminator or inhibitor probe in screening assays for novel antiviral compounds targeting viral replication.
• Biochemical Reagent Manufacturing: Sourced as a high-purity raw material for producing specialized kits and reagent mixes for molecular biology.

Basic Information

Product Name	1-(3'-C-Methyl-β-D-Ribofuranosyl)Uracil 5'-Triphosphate
CAS No.	87215-07-4
Molecular Formula	C11H17N2O15P3
Molecular Weight	534.17 g/mol
Synonyms	2'-C-Methyluridine-5'-triphosphate; 2'-C-Me-UTP; 1-(2-C-Methyl-β-D-ribofuranosyl)uracil 5'-triphosphate; 2'-Methyl-UTP; 2'-C-Methyl UTP; (2'R)-2'-C-Methyluridine 5'-triphosphate; 2'-C-Methyluridine triphosphate; β-2'-C-Methyluridine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our 1-(3'-C-Methyl-β-D-Ribofuranosyl)Uracil 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to rigorous analytical standards, utilizing HPLC, NMR, and MS for characterization, ensuring compliance with the stringent requirements of pharmaceutical R&D and molecular biology.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This compound is hygroscopic (moisture-sensitive). For long-term storage, desiccate under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Purity (NMR)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, slightly soluble in methanol
Biological Activity	Suitable for enzymatic incorporation (tested)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.