Description

Datp-α-S, [35S] is a radiolabeled nucleotide analog, specifically a sulfur-35 labeled deoxyadenosine triphosphate derivative. This compound is a critical reagent for sensitive detection and quantification in molecular biology research, enabling autoradiography and other radioisotopic tracing techniques. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers for applications such as DNA sequencing, nucleic acid hybridization, and enzyme activity assays.

Application

• Molecular Biology Research: As a labeled substrate for DNA polymerases in sequencing, primer extension, and labeling reactions.
• Nucleic Acid Hybridization: Used to prepare high-specific-activity radioactive probes for Southern, Northern, and dot-blot analyses.
• Enzyme Kinetics Studies: Employed in assays to study the activity and inhibition of kinases, polymerases, and other nucleotide-processing enzymes.
• Autoradiography: Provides a sensitive detection method for biomolecules separated by gel electrophoresis or immobilized on membranes.
• Metabolic Pathway Tracing: Useful in research investigating nucleotide metabolism and incorporation in cellular systems.
• Diagnostic Development: Serves as a key component in the research and development of radioactive diagnostic assays and kits.

Basic Information

Product Name	Datp-α-S, [35S]
CAS No.	87092-22-6
Molecular Formula	C₁₀H₁₆N₅O₁₂P₃S * (³⁵S)
Molecular Weight	~507.2 g/mol (unlabeled)
Synonyms	2'-Deoxyadenosine-5'-O-(1-thiotriphosphate), [α-³⁵S]; Adenosine 5'-(γ-thio)triphosphate, 2'-deoxy-, [α-³⁵S]; dATPαS-[³⁵S]; [α-³⁵S]dATPαS; 2'-Deoxyadenosine 5'-(α-thiotriphosphate), sulfur-35 labeled; Radiolabeled dATPαS; Thio-dATP-[³⁵S]; Deoxyadenosine triphosphate, α-thio, S-35 labeled
EINECS	Contact for details

Quality Control

Every batch of Datp-α-S, [35S] is manufactured and controlled to meet the stringent requirements of research use. Quality is assured through rigorous analytical testing, including HPLC for radiochemical purity and identity confirmation. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing the specific activity, purity, and analytical results. Our quality system ensures consistency and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For optimal stability and to minimize radiolysis, store frozen at or below -20°C in the original lead-shielded container. Avoid repeated freeze-thaw cycles. Handle and dispose of in accordance with all applicable local, national, and international radiation safety regulations.

Specification

Item	Specification
Appearance	Clear solution
Identification (HPLC)	Conforms
Specific Activity	>1000 Ci/mmol
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC)	≥ 90%
Solvent	Aqueous buffer solution
Concentration	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.