Description

n-p-Tosyl-Gly-Pro-Arg p-Nitroanilide Acetate Salt is a high-purity chromogenic substrate specifically designed for the detection and quantification of thrombin and other serine proteases. This compound is critical for biochemical research and diagnostic applications where precise enzymatic activity measurement is required. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions focused on hemostasis, thrombosis, and protease inhibitor studies.

Application

• Thrombin Activity Assays: Primary substrate for the colorimetric determination of thrombin activity in plasma samples and purified systems.
• Diagnostic Kit Manufacturing: A key reagent in the production of clinical diagnostic kits for monitoring coagulation disorders and anticoagulant therapy.
• Serine Protease Research: Used in biochemical studies to investigate the kinetics and inhibition profiles of trypsin-like serine proteases.
• Drug Discovery & Screening: Essential tool in high-throughput screening (HTS) platforms for identifying and characterizing novel anticoagulant and antithrombotic drug candidates.
• Quality Control in Biologics: Employed to test for residual protease activity in the downstream processing of therapeutic proteins.
• Academic & Clinical Research: Supports fundamental research into blood coagulation pathways, inflammatory processes, and related disease mechanisms.

Basic Information

Product Name	n-p-Tosyl-Gly-Pro-Arg p-Nitroanilide Acetate Salt
CAS No.	86890-95-1
Molecular Formula	C28H37N9O9S • xC2H4O2
Molecular Weight	675.73 g/mol (Free base)
Synonyms	Tos-Gly-Pro-Arg-pNA Acetate Salt; Chromozym TH; H-D-Phe-Pip-Arg-pNA • 2HCl; Thrombin Substrate I; S-2238; Benzoyl-Phe-Val-Arg p-nitroanilide hydrochloride (related); p-Tosyl-Gly-Pro-Arg-p-Nitroanilide; GPR-pNA Acetate
EINECS	Contact for details

Quality Control

Our n-p-Tosyl-Gly-Pro-Arg p-Nitroanilide Acetate Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data and specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Clear, colorless solution in water or DMSO
Functional Activity	≥ 95% of reference standard in thrombin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.