Description

n-(4'-Azido-2'-Nitrophenyl)-12-Aminododecanoyl-Coenzyme A is a specialized, chemically modified Coenzyme A derivative designed for advanced biochemical research and labeling applications. This compound matters for its unique bifunctional structure, featuring a photoreactive azido group and a long-chain aminododecanoyl linker, enabling precise cross-linking and affinity tagging of biomolecules. It is primarily needed by research institutions and pharmaceutical R&D departments focused on proteomics, enzyme mechanism studies, and the development of novel diagnostic assays.

Application

• Enzyme Activity Profiling and Inhibition Studies: Used as a mechanism-based probe to covalently label and identify active sites of target enzymes.
• Affinity-Based Protein Purification: Serves as a key intermediate in the synthesis of immobilized ligands for affinity chromatography columns.
• Proteomics and Biomarker Discovery: Employed in photoaffinity labeling experiments to capture and characterize transient protein-cofactor interactions.
• Metabolic Pathway Analysis: Acts as a tracer or inhibitor in studies investigating acyl-CoA dependent metabolic pathways.
• Diagnostic Reagent Development: Utilized in the creation of sensitive detection kits for specific enzymatic activities.
• Bioconjugation and Chemical Biology: The azido group allows for subsequent "click chemistry" reactions, facilitating the attachment of fluorophores or other reporter molecules.

Basic Information

Product Name	n-(4'-Azido-2'-Nitrophenyl)-12-Aminododecanoyl-Coenzyme A
CAS No.	86450-73-9
Molecular Formula	C38H61N8O19P3S
Molecular Weight	1050.93 g/mol
Synonyms	12-[(4-Azido-2-nitrophenyl)amino]dodecanoyl-CoA; ANPAD-CoA; 4-Azido-2-nitrophenylaminododecanoyl Coenzyme A; CoA-ANPAD; Photoactivatable Coenzyme A Derivative; 4-Azido-2-nitroanilino-dodecanoyl-CoA; N-(4-Azido-2-nitrophenyl)-12-aminododecanoyl-CoA; 86450-73-9
EINECS	Contact for details

Quality Control

Our n-(4'-Azido-2'-Nitrophenyl)-12-Aminododecanoyl-Coenzyme A is produced under stringent conditions to ensure batch-to-batch consistency and high purity, critical for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and spectroscopic methods for structural confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request, supporting compliance with rigorous research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. Due to its hygroscopic and strictly light-sensitive nature, this material should be handled under inert atmosphere when possible and aliquoted to minimize repeated freeze-thaw cycles. Keep container tightly sealed in a dry, dark place.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Conforms
Identification (UV-Vis)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, Methanol, Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.