Description

Biotin-16-Deoxyuridine-5'-Triphosphate, Lithium Salt - 1 Mm Aqueous Solution is a chemically modified nucleotide analog designed for sensitive detection and labeling applications. This product matters as a critical reagent for the specific, high-affinity tagging of nucleic acids, enabling advanced molecular biology and diagnostic research. It is primarily needed by biotechnology, pharmaceutical, and academic research sectors for applications such as non-radioactive probe labeling, in situ hybridization, and next-generation sequencing workflows.

Application

• Non-Radioactive Nucleic Acid Labeling: Acts as a substrate for enzymatic incorporation into DNA probes for blotting and microarray applications.
• In Situ Hybridization (ISH): Used to generate labeled probes for the detection of specific DNA or RNA sequences within cells and tissues.
• Next-Generation Sequencing (NGS): Serves as a modified nucleotide for specialized sequencing-by-synthesis protocols and library preparation.
• Molecular Diagnostics: Enables the development of highly sensitive diagnostic assays and detection kits.
• Bioconjugation & Probe Development: Facilitates the creation of biotinylated oligonucleotides for capture or detection via streptavidin-biotin interaction.
• DNA/RNA Modification Studies: A tool for investigating nucleotide metabolism, DNA repair mechanisms, and polymerase activity.

Basic Information

Item	Detail
Product Name	Biotin-16-Deoxyuridine-5'-Triphosphate, Lithium Salt - 1 Mm Aqueous Solution
CAS No.	86303-26-6
Molecular Formula	C₃₁H₄₃Li₃N₅O₁₉P₃
Molecular Weight	Contact for details
Synonyms	Biotin-16-dUTP, Li Salt; 5'-Biotin-16-deoxyuridine-triphosphate; Biotinylated dUTP; Biotin-16-2'-deoxyuridine-5'-triphosphate lithium salt; 5-(Biotin-16-yl)-2'-deoxyuridine-5'-triphosphate; 2'-Deoxy-5-(N-(biotinoyl-16-amidocaproyl)aminopropyl)uridine 5'-triphosphate; Biotin-16-allylamino-dUTP; Biotin-XX-dUTP (common trade name style)
EINECS	Contact for details

Quality Control

Our Biotin-16-Deoxyuridine-5'-Triphosphate, Lithium Salt solution is manufactured under stringent conditions to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous quality testing, including HPLC analysis for purity and identity verification, to ensure compliance with research-grade standards. Certificates of Analysis (COA) detailing purity, concentration, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is easily oxidized; it is recommended to store under an inert atmosphere when possible and aliquot upon receipt to minimize headspace exposure.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow aqueous solution
Concentration	1 mM ± 10%
Purity (HPLC)	≥ 95%
Identification (IR/UV)	Conforms to reference standard
pH	6.5 - 7.5
Functional Activity	Suitable for enzymatic incorporation (e.g., by Terminal Transferase)
Biotin Content	≥ 0.9 mol biotin / mol nucleotide
Endotoxin Level	< 0.1 EU/µmol (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.