Description

4-Diazophenylgalactoside is a specialized biochemical reagent primarily used as a chromogenic substrate in enzymatic assays. This compound is valued for its role in detecting and quantifying the activity of specific enzymes, such as β-galactosidase, through the release of a colored diazonium product. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic kit manufacturing sectors who require precise and sensitive detection methods.

Application

• Enzyme Activity Assays: As a chromogenic substrate for β-galactosidase in molecular biology and biochemistry research.
• Diagnostic Reagent Manufacturing: Used in the production of test kits for clinical diagnostics and point-of-care testing.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify enzyme inhibitors or activators.
• Cell & Tissue Staining: Utilized in histochemistry and reporter gene assays (e.g., lacZ) for visualizing gene expression.
• Biochemical Research: A key reagent for studying glycosidase enzyme kinetics and mechanisms.
• Quality Control Testing: Applied in the QC of recombinant protein production and enzyme purification processes.

Basic Information

Product Name	4-Diazophenylgalactoside
CAS No.	86277-63-6
Molecular Formula	C12H14N2O6
Molecular Weight	282.25 g/mol
Synonyms	4-Diazophenyl β-D-galactopyranoside; p-Diazophenylgalactoside; Galactoside, 4-diazophenyl; Diazophenyl galactoside; 4-DPG; Chromogenic β-galactosidase substrate; p-Diazoniumphenyl-β-D-galactopyranoside; 4-Diazoniophenyl β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our 4-Diazophenylgalactoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content	≤ 2.0%
Solubility	Soluble in water, DMSO, and buffer solutions
Functional Activity	Suitable for β-galactosidase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.