Description

Pyro-Glu-Pro-Val-Pna is a specialized peptide derivative, specifically a pyroglutamyl tripeptide p-nitroanilide substrate. This compound is valued for its role as a highly specific and sensitive enzymatic substrate in biochemical research and diagnostic development. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and clinical diagnostics sectors for enzyme activity assays and inhibitor screening.

Application

• Enzymology Research: As a chromogenic substrate for studying the kinetics and specificity of proteolytic enzymes, particularly those cleaving at proline residues.
• Diagnostic Assay Development: Used in the formulation of colorimetric test kits for detecting and quantifying specific enzyme activities in clinical samples.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for drug discovery.
• Biochemical Reagent: Serves as a critical component in standardized laboratory protocols for measuring peptidase or protease activity.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme-based products or processes.

Basic Information

Product Name	Pyro-Glu-Pro-Val-Pna
CAS No.	86190-22-9
Molecular Formula	C22H29N7O7
Molecular Weight	503.51 g/mol
Synonyms	Pyr-Glu-Pro-Val-pNA; pGlu-Pro-Val-p-Nitroanilide; L-Pyroglutamyl-L-prolyl-L-valine p-nitroanilide; Pyroglutamyl-Prolyl-Valyl-pNA; Suc-GPV-pNA; Substrate for DPP IV; DPP IV Substrate; 5-Oxo-L-prolyl-L-prolyl-L-valine 4-nitroanilide
EINECS	Contact for details

Quality Control

Our Pyro-Glu-Pro-Val-Pna is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.