Description

n-Cbz-Val-Gly-Arg p-Nitroanilide Acetate Salt is a specialized chromogenic peptide substrate designed for the sensitive and specific detection of proteolytic enzyme activity. This compound is critical for biochemical research and diagnostic development, where precise measurement of enzyme kinetics is required. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, clinical diagnostics, and life science research.

Application

• Protease Activity Assays: As a chromogenic substrate for the detection and quantification of trypsin, thrombin, and other serine proteases.
• Biochemical Research: Used in enzyme kinetics studies to determine Michaelis-Menten constants (Km) and catalytic efficiency (kcat).
• Diagnostic Kit Development: A key component in the formulation of diagnostic tests for blood coagulation disorders and other protease-related pathologies.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Quality Control in Biologics: Used to monitor and validate the activity of protease enzymes used in biomanufacturing processes.

Basic Information

Product Name	n-Cbz-Val-Gly-Arg p-Nitroanilide Acetate Salt
CAS No.	86170-43-6
Molecular Formula	C28H38N8O8 • C2H4O2
Molecular Weight	658.69 g/mol
Synonyms	Benzyloxycarbonyl-Val-Gly-Arg p-Nitroanilide Acetate; Z-Val-Gly-Arg-pNA Acetate; CBZ-Val-Gly-Arg-pNA Acetate; Nα-Carbobenzoxy-L-valylglycyl-L-arginine 4-nitroanilide acetate; Chromogenic Substrate for Trypsin; S-2765 (related analog); Chromozym TRY (related analog); Protease Substrate 86170-43-6
EINECS	Contact for details

Quality Control

Our n-Cbz-Val-Gly-Arg p-Nitroanilide Acetate Salt is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We adhere to relevant industry standards for research-grade biochemicals to support your critical R&D and manufacturing needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Clear, colorless solution in DMSO or DMF
Functional Activity	Cleavage by trypsin yields p-nitroaniline (pNA) as confirmed by absorbance at 405 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.