Description

5'-(((4-Azidophenacyl)Thio)Phosphoryl)Adenylyl(3'-5')Uridine is a specialized, chemically modified dinucleotide analog designed for advanced biochemical research and development. This compound is a critical tool for photoaffinity labeling and cross-linking studies, enabling the investigation of RNA-protein interactions and nucleic acid structure. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies focused on molecular biology, structural biology, and drug discovery.

Application

• Photoaffinity Labeling Probe: Acts as a key reagent for covalently tagging and identifying RNA-binding proteins and other biomolecular targets upon UV light activation.
• Structural Biology Research: Used in cross-linking experiments to map RNA tertiary structures and study conformational dynamics.
• Bioconjugation and Immobilization: Serves as a versatile intermediate for attaching RNA sequences to solid supports or other molecules via the reactive azido group.
• Mechanistic Studies: Facilitates the elucidation of enzymatic mechanisms in RNA processing, splicing, and translation.
• Drug Discovery Screening: Employed in high-throughput assays to discover and characterize novel compounds that interact with specific RNA motifs.
• Diagnostic Assay Development: Used in the design of advanced diagnostic platforms that rely on specific nucleic acid capture and detection.

Basic Information

Product Name	5'-(((4-Azidophenacyl)Thio)Phosphoryl)Adenylyl(3'-5')Uridine
CAS No.	85977-19-1
Molecular Formula	C27H28N10O15P2S
Molecular Weight	850.59 g/mol
Synonyms	5'-(((4-Azidophenacyl)Thio)Phosphoryl)Adenylyl(3'-5')Uridine; 4-Azidophenacyl Thio Phosphoryl Adenylyl Uridine; ApU Photoaffinity Labeling Analog; N6-[(4-Azidophenacyl)thio]phosphoryl-adenylyl-uridine; 5'-O-[N-(4-Azidophenacyl)phosphoramidothioate]-adenylyl-(3'→5')-uridine; Photoactivatable ApU Dinucleotide; CAS 85977-19-1
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research-grade chemicals. Every batch is characterized using advanced analytical techniques including HPLC, mass spectrometry (MS), and NMR to confirm identity, purity, and stability. Certificates of Analysis (COA) detailing all specifications are available upon request, providing full traceability and supporting your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This compound is light-sensitive (store away from light) and moisture-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize condensation. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.