Description

p-Nitrophenyl 4,6-Benzylidene-α-D-Glucopyranoside is a specialized synthetic glycoside derivative, primarily utilized as a chromogenic substrate in enzymatic assays. This compound is critical for research and development in biochemistry and molecular biology, enabling the precise measurement of glycosidase enzyme activity. It is an essential reagent for pharmaceutical research, diagnostic kit manufacturing, and academic laboratories focused on carbohydrate metabolism and enzyme kinetics.

Application

• Enzymatic Substrate for Glycosidases: Acts as a chromogenic substrate for α-glucosidase and related enzymes, producing a yellow-colored p-nitrophenol upon hydrolysis for quantitative analysis.
• Pharmaceutical Research & High-Throughput Screening (HTS): Used in drug discovery to identify and characterize inhibitors of carbohydrate-processing enzymes for therapeutic development.
• Diagnostic Reagent Manufacturing: Incorporated into clinical diagnostic kits for the detection of enzymatic deficiencies or specific metabolic disorders.
• Biochemical Research & Enzyme Kinetics: Employed in academic and industrial labs to study enzyme mechanisms, specificity, and catalytic efficiency.
• Quality Control in Biotechnology: Serves as a standard reagent for the quality assurance of enzyme preparations and biocatalysts.

Basic Information

Product Name	p-Nitrophenyl 4,6-Benzylidene-α-D-Glucopyranoside
CAS No.	85906-27-0
Molecular Formula	C19H19NO8
Molecular Weight	389.36 g/mol
Synonyms	4-Nitrophenyl 4,6-O-benzylidene-α-D-glucopyranoside; p-Nitrophenyl 4,6-O-benzylidene-α-D-glucoside; 4,6-O-Benzylidene-α-D-glucopyranosyl p-nitrophenyl ether; α-D-Glucopyranoside, p-nitrophenyl 4,6-O-benzylidene-; PNPG-benzylidene; Chromogenic α-glucosidase substrate
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 4,6-Benzylidene-α-D-Glucopyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards of research and diagnostic industries.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.