Description

Insulin, (2-Nitro-4-Azidophenyl)-Gly(B29)- is a chemically modified insulin derivative, specifically labeled at the B29 glycine residue with a photoactivatable aryl azide group. This compound is a critical research-grade biochemical tool designed for photoaffinity labeling studies, enabling the covalent cross-linking and identification of insulin receptor binding sites and interacting proteins. It is essential for researchers in pharmaceutical development, biochemistry, and molecular biology who require precise probes for investigating insulin's mechanism of action, receptor mapping, and signal transduction pathways. The product is supplied as a high-purity, lyophilized solid to ensure optimal performance in sensitive experimental applications.

Application

• Photoaffinity Labeling Studies: Primary application for covalent tagging and identification of insulin receptor subunits and binding domains.
• Biochemical Research: Used as a molecular probe to study insulin-protein interactions, signal transduction mechanisms, and metabolic pathways.
• Drug Discovery & Development: Facilitates target validation and the study of novel insulin mimetics or receptor modulators in pre-clinical research.
• Structural Biology: Aids in mapping the three-dimensional interaction interface between insulin and its receptor using cross-linking techniques.
• Diagnostic Assay Development: Can be utilized in the development of advanced immunoassays or biosensors for insulin detection and characterization.

Basic Information

Product Name	Insulin, (2-Nitro-4-Azidophenyl)-Gly(B29)-
CAS No.	85764-63-2
Molecular Formula	C257H387N65O79S6
Molecular Weight	Approx. 5828.5 g/mol
Synonyms	NεB29-(2-Nitro-4-azidophenylacetyl)insulin; 2-Nitro-4-azidophenyl-Gly(B29)-insulin; Photoactivatable Insulin Derivative; Insulin aryl azide; Insulin photoreactive probe; B29-Glycine modified insulin; Insulin, Nε-(2-nitro-4-azidophenylacetyl)-L-lysinyl-; Insulin (2-nitro-4-azidophenyl)glycine B29 derivative
EINECS	Contact for details

Quality Control

Our Insulin, (2-Nitro-4-Azidophenyl)-Gly(B29)- is manufactured under controlled conditions to ensure high batch-to-batch consistency and purity suitable for research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and mass spectrometry for identity confirmation. We provide comprehensive Certificates of Analysis (COA) detailing all specifications, including purity, biological activity where applicable, and absence of key impurities. Our quality commitment ensures the material meets the stringent requirements of advanced biochemical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to light brown lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Biological Activity	Contact for details
Single Impurity (HPLC)	≤ 1.0 %
Solubility	Soluble in DMSO, dilute acetic acid

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.